Pérez-Ruiz M, Rodríguez-Granger J M, Bautista-Marín M F, Romero-Noguera J, Rosa-Fraile M
Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avenida Fuerzas Armadas, s/n 18014, Granada, Spain.
Epidemiol Infect. 2004 Apr;132(2):375-8. doi: 10.1017/s0950268803001845.
Pulsed-field gel electrophoresis (PFGE) of SmaI-DNA digests and serotyping was performed on 15 colonies of Streptococcus agalactiae (GBS) from each of 30 vaginal rectal colonized women. Five distinct GBS serotypes were observed among the 30 specimens (Ia, Ib, II, III and V). In 29 of the 30 samples, the same serotype was observed among all 15 colonies; in the remaining specimen, the 15 colonies yielded two serotypes (II and V). The PFGE profiles of all colonies in 27 of the 30 subjects were indistinguishable within each subject. In the remaining women, different DNA profiles were identified among the colonies in each specimen, one of whom carried two different serotypes. Furthermore, strains of the same serotype belonging to different women were genetically heterogeneous.
对30名阴道直肠定植的女性中每人分离出的15株无乳链球菌（GBS）菌落进行了SmaI - DNA酶切片段的脉冲场凝胶电泳（PFGE）和血清分型。在30份标本中观察到5种不同的GBS血清型（Ia、Ib、II、III和V）。在30个样本中的29个样本中，所有15个菌落的血清型均相同；在其余标本中，15个菌落产生了两种血清型（II和V）。30名受试者中27名受试者的所有菌落的PFGE图谱在个体内无法区分。在其余女性中，每个标本中的菌落鉴定出不同的DNA图谱，其中一人携带两种不同的血清型。此外，来自不同女性的相同血清型菌株在基因上是异质的。
