Savoia D, Gottimer C, Crocilla' C, Zucca M
Department of Clinical and Biological Sciences, University of Turin, at S. Luigi Gonzaga Hospital, Regione Gonzole 10, 10043 Orbassano (TO), Italy.
J Infect. 2008 Feb;56(2):120-5. doi: 10.1016/j.jinf.2007.11.007. Epub 2007 Dec 31.
Streptococcus agalactiae (GBS) is considered a leading cause of neonatal sepsis. We evaluated the phenotypic and genotypic characters of 73 S. agalactiae strains isolated from different women at the 35-37 weeks of pregnancy.
Isolates were characterized by serotyping (direct agglutination) and by pulsed-field-gel-electrophoresis (PFGE). Resistance to antimicrobials (penicillin, macrolides, lincosamides, quinolones and tetracyclines) was assessed.
All isolates were serologically typeable and ascribable to one of the six tested serotypes (Ia, Ib, II, III, IV, and V) and many strains of the same serotype were genetically heterogeneous. Strains belonging to serotypes III, V and Ia were the most prevalent and the most resistant to macrolides.
This work reports GBS colonization rate (about 18%) and the prevalent capsular serotypes among pregnant women in Turin (Italy). Penicillin and erythromycin can be still considered the first and second choice drugs for prophylaxis and treatment of early-onset GBS infections in our district. The relevance of phenotypic and genotypic characterization of strains to monitor and control Streptococcus agalactiae infections is briefly discussed.
无乳链球菌（GBS）被认为是新生儿败血症的主要病因。我们评估了从不同孕妇在妊娠35 - 37周时分离出的73株无乳链球菌菌株的表型和基因型特征。
通过血清分型（直接凝集法）和脉冲场凝胶电泳（PFGE）对分离株进行特征分析。评估其对抗菌药物（青霉素、大环内酯类、林可酰胺类、喹诺酮类和四环素类）的耐药性。
所有分离株均可进行血清分型，可归为六种测试血清型（Ia、Ib、II、III、IV和V）之一，且许多相同血清型的菌株在基因上具有异质性。血清型III、V和Ia的菌株最为常见，且对大环内酯类耐药性最强。
本研究报告了意大利都灵孕妇中GBS的定植率（约18%）及流行的荚膜血清型。在我们地区，青霉素和红霉素仍可被视为预防和治疗早发性GBS感染的首选和次选药物。简要讨论了菌株的表型和基因型特征对于监测和控制无乳链球菌感染的相关性。
