Sakulwira Kamol, Theamboonlers Apiradee, Oraveerakul Kanisak, Chaiyabutr Narongsak, Bhattarakosol Parvapan, Poovorawan Yong
Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
J Med Primatol. 2004 Feb;33(1):25-9. doi: 10.1111/j.1600-0684.2003.00049.x.
A male orangutan suffered from ulcers at the buccal mucosa. We obtained swab fluid from the base of both vesicles and ulcers and collected blood for further separation into serum, plasma and peripheral blood mononuclear cells (PBMC) for detection of antibody to herpesvirus by serology and herpesvirus DNA by polymerase chain reaction (PCR) using consensus degenerate primers. Serology was positive for human EBV IgG but negative for Epstein-Barr virus (EBV) immunoglobulin (IgM), as well as for both human cytomegalovirus and herpes simplex virus IgG and IgM. Upon PCR, we obtained a 232-bp product of virus DNA from PBMC, but not from lesions, serum or plasma. We confirmed the positive result by direct sequencing and compared the nucleotide sequence with other nucleotide sequences applying the BLAST program from GenBank. The sequence was similar to lymphocryptovirus of macaque (93%), marmoset (93%), gorilla (90%) and human EBV (90%). We aligned this sequence with other sequences in GenBank and performed phylogenetic analysis, showing that it probably belongs to the gammaherpesvirus group.
一只雄性猩猩的颊黏膜出现溃疡。我们从水疱和溃疡底部获取了拭子样本，并采集血液以便进一步分离出血清、血浆和外周血单核细胞（PBMC），通过血清学检测疱疹病毒抗体，并用共识简并引物通过聚合酶链反应（PCR）检测疱疹病毒DNA。血清学检测显示人类EBV IgG呈阳性，但爱泼斯坦-巴尔病毒（EBV）免疫球蛋白（IgM）呈阴性，人类巨细胞病毒和单纯疱疹病毒的IgG和IgM也均为阴性。PCR检测时，我们从PBMC中获得了一段232 bp的病毒DNA产物，但在病变组织、血清或血浆中未获得。我们通过直接测序确认了阳性结果，并使用GenBank的BLAST程序将核苷酸序列与其他核苷酸序列进行了比较。该序列与猕猴的淋巴细胞隐病毒（93%）、狨猴（93%）、大猩猩（90%）和人类EBV（90%）相似。我们将该序列与GenBank中的其他序列进行比对并进行系统发育分析，结果表明它可能属于γ疱疹病毒组。
