圈养红毛猩猩和长臂猿中乙型肝炎病毒的流行率、全基因组特征及系统发育分析
Prevalence, whole genome characterization and phylogenetic analysis of hepatitis B virus in captive orangutan and gibbon.
作者信息
Sa-nguanmoo Pattaratida, Thongmee Chittima, Ratanakorn Parntep, Pattanarangsan Rattapan, Boonyarittichaikij Roschong, Chodapisitkul Somchai, Theamboonlers Apiradee, Tangkijvanich Pisit, Poovorawan Yong
机构信息
Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
出版信息
J Med Primatol. 2008 Dec;37(6):277-89. doi: 10.1111/j.1600-0684.2008.00290.x. Epub 2008 May 2.
BACKGROUND
Hepatitis B virus (HBV) is a public health problem worldwide and apart from infecting humans, HBV has been found in non-human primates.
METHODS
We subjected 93 non-human primates comprising 12 species to ELISA screening for the serological markers HBsAg, antiHBs and antiHBc. Subsequently, we detected HBV DNA, sequenced the whole HBV genome and performed phylogenetic analysis.
RESULTS
HBV infection was detected in gibbon (4/15) and orangutan (7/53). HBV DNA isolates from two gibbons and seven orangutans were chosen for complete genome amplification. We aligned the Pre-S/S, Pre-C/C and entire genomes with HBV sequences and performed phylogenetic analysis. The gibbon and orangutan viruses clustered within their respective groups.
CONCLUSIONS
Both geographic location and host species influence which HBV variants are found in gibbons and orangutans. Hence, HBV transmission between humans and non-human primates might be a distinct possibility and additional studies will be required to further investigate this potential risk.
背景
乙型肝炎病毒（HBV）是一个全球性的公共卫生问题，除了感染人类外，还在非人类灵长类动物中被发现。
方法
我们对包括12个物种的93只非人类灵长类动物进行了ELISA检测，以筛查血清学标志物HBsAg、抗-HBs和抗-HBc。随后，我们检测了HBV DNA，对整个HBV基因组进行了测序，并进行了系统发育分析。
结果
在长臂猿（4/15）和猩猩（7/53）中检测到HBV感染。从两只长臂猿和七只猩猩中分离出的HBV DNA被用于全基因组扩增。我们将前S/S、前C/C和整个基因组与HBV序列进行比对，并进行了系统发育分析。长臂猿和猩猩病毒聚集在各自的组内。
结论
地理位置和宿主物种都会影响在长臂猿和猩猩中发现的HBV变体。因此，人类与非人类灵长类动物之间的HBV传播很有可能发生，需要进一步的研究来进一步调查这种潜在风险。
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