Offei S K, Asante I K, Danquah E Y
Molecular Biology Laboratory, Department of Crop Science, University of Ghana, Legon, Ghana.
Hereditas. 2004;140(2):123-8. doi: 10.1111/j.1601-5223.2004.01725.x.
Seventy cocoyam accessions collected from the eastern and Volta regions of Ghana were studied using RAPD technique. Ten primers were used to study the genetic diversity and structure of the experimental material and a total of 120 different bands were detected. Levels of polymorphic fragments detected by the ten primers ranged from 69.2% to 100%. In the accessions from the eastern region, the mean observed and effective number of alleles per individual per population and mean Nei's gene diversity were 1.99+/-0.01, 1.53+/-0.12 and 0.312+/-0.05, respectively. Mean observed and effective number of alleles per individual per population and Nei's gene diversity were 1.76+/-0.17, 1.44+/-0.16 and 0.264+/-0.08, respectively for the accessions from the Volta region. Considering the entire collections, the mean observed and effective number of alleles per individual per population and Nei's gene diversity for primers were 1.99+/-0.02, 1.54+/-0.12 and 0.319+/-0.05, respectively. In terms of Nei's F-statistics in the subdivided populations for all primers, the overall gene diversity (Ht) ranged from 0.230 to 0.396 with a mean of 0.313+0.06, within sample gene diversity (Hs) ranged from 0.214 to 0.372 with a mean of 0.286+0.06, gene differentiation (Gst) ranged between 0.005 and 0.178 with a mean of 0.086. Geneflow estimate ranged between 2.309 and 99.500 with a mean of 5.314. The accessions grouped into three main clusters. Accession BD96/183 was the most diverse and may be incorporated into cocoyam breeding programs. The 70 accessions did not cluster into their distinct geographical regions suggesting that there may have been movement of germplasm across the two regions.
利用随机扩增多态性DNA（RAPD）技术对从加纳东部和沃尔特地区收集的70份芋种质进行了研究。使用10种引物来研究实验材料的遗传多样性和结构，共检测到120条不同的条带。10种引物检测到的多态性片段水平在69.2%至100%之间。在来自东部地区的种质中，每个群体中每个个体的平均观察等位基因数和有效等位基因数以及平均奈氏基因多样性分别为1.99±0.01、1.53±0.12和0.312±0.05。对于来自沃尔特地区的种质，每个群体中每个个体的平均观察等位基因数和有效等位基因数以及奈氏基因多样性分别为1.76±0.17、1.44±0.16和0.264±0.08。考虑到整个收集物，引物的每个群体中每个个体的平均观察等位基因数和有效等位基因数以及奈氏基因多样性分别为1.99±0.02、1.54±0.12和0.319±0.05。就所有引物在细分群体中的奈氏F统计量而言，总体基因多样性（Ht）在0.230至0.396之间，平均值为0.313±0.06，样本内基因多样性（Hs）在0.214至0.372之间，平均值为0.286±0.06，基因分化（Gst）在0.005至0.178之间，平均值为0.086。基因流估计值在2.309至99.500之间，平均值为5.314。这些种质分为三个主要聚类。种质BD96/183的多样性最高，可纳入芋育种计划。这70份种质并未聚类到其各自不同的地理区域，这表明两个地区之间可能存在种质流动。
