[金荞麦遗传多样性的随机扩增多态性DNA分析]

[RAPD analysis for genetic diversity of Fagopyrum cymosum].

作者信息

Zhang Chunping, He Ping, Hu Shijun, Zhang Yifeng, Ji Huacun, Gao Shan

机构信息

School of Life Sciences, Southwest University, Key Laboratory (Ministry of Education) of Eco-environments of Three Gorges Reservoir Region, Chongqing Key Laboratory of Plant Ecology , Chongqing, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Mar;34(6):660-3.

PMID:19630185

Abstract

OBJECTIVE

To study the genetic diversity of Fagopyrum cymosum.

METHOD

The genetic diversity of 87 individuals from 10 populations was analyzed by random amplified polymorphic DNA (RAPD).

RESULT

Twelve primers were selected to produce highly reproducible RAPD bands. Among 85 amplified bands, seventy-five showed polymorphism, the percentage of polymorphic bands reached to 88.24%. Nei's gene diversity index (H) was 0. 3103, Shannon information index (I) was 0.5632, Gst was 0.1924. The genetic similarity coefficient and the genetic distance were 0.6720-0.9678 and 0.0328-0.3975, respectively.

CONCLUSION

F. cymosum shows high genetic diversity and the majority of genetic variation occurs in populations. By cluster analysis, the geographical distribution is very obvious. The RAPD marker can be used for the analysis of the genetic diversity and genetic variation of F. cymosum.

摘要

目的

研究金荞麦的遗传多样性。

方法

采用随机扩增多态性DNA（RAPD）技术对10个居群的87个个体进行遗传多样性分析。

结果

筛选出12条引物，扩增出的RAPD条带重复性好。85条扩增条带中，75条具有多态性，多态性条带比例达88.24%。Nei氏基因多样性指数（H）为0.3103，Shannon信息指数（I）为0.5632，基因分化系数（Gst）为0.1924。遗传相似系数和遗传距离分别为0.6720 - 0.9678和0.0328 - 0.3975。