Ma Cai-ling, Li Yi-jie, Zhang Fu-chun, Wang Guo-quan, Zheng Yu-jian, Kai Li-man, Re Xi-dan, Han Ying
Department of Obstetrics and Gynecology, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China.
Zhonghua Yi Xue Za Zhi. 2004 Mar 17;84(6):469-73.
To establish a method for detection of Human Papillomavirus (HPV) type16 E6 gene in Cervical carcinomas Specimens. To study the relationship between the quantities of HPV16 E6 (Human papillomavirus type16 E6 gene) in cervical tissues and the course of cervical disease in Xinjiang.
HPV16E6 gene and beta-actin was detected in parallel by FQ-PCR (fluorescence quantitative PCR). The number of copies of HPV16 E6 gene and beta-actin was detected in parallel by FQ-PCR (fluorescence quantitative PCR) in tissues of 69 cervical cancer, 65 cervical intraepithelial neoplasia (CIN), 33chronic cervicitis and samples of 96 cervical smear samples of vaginitis and cervicitis. The variation in HPV copies per genomic DNA equivalent can be estimated by dividing the HPV copy number by the beta-actin copy number.
The positive rate of HPV16 E6 gene was 83.0%, 75.7%, 93.3% and 3.3% in tissues of cervical cancer, cervical intraepithelial neoplasia (CIN), chronic cervicitis and samples of cervical smear respectively. The amount of HPV16 E6 gene was gradually higher by the developing of the course of cervical disease. They have positive rank correlation, r = 0.83, P < 0.01.
The study underscores the importance of the relationship between the HPV16 E6 gene and the course of cervical disease in Xinjiang. It also suggests that the quantification of HPV16 E6 gene may be useful as a prognostic tool to identify women who are at increased risk of developing cervical cancer. This method may be applied to studies of a number of issues related to the natural history of cervical cancer, such as the amounts of HPV in high- and low-grade lesions.
建立一种检测宫颈癌标本中16型人乳头瘤病毒（HPV）E6基因的方法。研究新疆地区宫颈组织中HPV16 E6（16型人乳头瘤病毒E6基因）含量与宫颈疾病病程的关系。
采用荧光定量聚合酶链反应（FQ-PCR）同时检测HPV16 E6基因和β-肌动蛋白。运用FQ-PCR同时检测69例宫颈癌、65例宫颈上皮内瘤变（CIN）、33例慢性宫颈炎组织以及96例阴道炎和宫颈炎宫颈涂片样本中HPV16 E6基因和β-肌动蛋白的拷贝数。通过将HPV拷贝数除以β-肌动蛋白拷贝数，可估算每基因组DNA当量中HPV拷贝数的变化。
HPV16 E6基因在宫颈癌、宫颈上皮内瘤变（CIN）、慢性宫颈炎组织及宫颈涂片样本中的阳性率分别为83.0%、75.7%、93.3%和3.3%。随着宫颈疾病病程进展，HPV16 E6基因含量逐渐升高。二者呈正秩相关，r = 0.83，P < 0.01。
该研究强调了新疆地区HPV16 E6基因与宫颈疾病病程关系的重要性。这也表明HPV16 E6基因定量可能作为一种预后工具，用于识别患宫颈癌风险增加的女性。该方法可应用于许多与宫颈癌自然史相关问题的研究，如高低级别病变中的HPV含量。
