Langdon Jacqueline M, Vonakis Becky M, MacDonald Susan M
The Division of Allergy and Clinical Immunology, Asthma and Allergy Center, The Johns Hopkins School of Medicine, 5501 Hopkins Bayview Circle, Baltimore, MD 21224, USA.
Biochim Biophys Acta. 2004 Apr 5;1688(3):232-6. doi: 10.1016/j.bbadis.2003.12.007.
The human recombinant histamine releasing factor (HrHRF), also known as translationally controlled tumor protein (TCTP), p23 and fortilin, has been described to have both extra- and intracellular functions. To elucidate an extra- or intracellular role for HrHRF, we used the yeast two-hybrid system with HrHRF as the bait and a Jurkat T cell library. We isolated a partial cDNA clone of the human elongation factor-1 delta (EF-1delta) encoding for amino acids 12 to 281. This interaction was confirmed by co-immunoprecipitation experiments. Previously, both HrHRF and EF-1delta have been isolated and identified in association with malignancy in numerous studies. EF-1delta is part of the EF-1 complex responsible for kinetic proofreading in protein synthesis. Additionally, DNA microarray data classifies TCTP (HrHRF) as co-regulated with ribosomal proteins and recent structural analysis of TCTP (HrHRF) relates it to a guanine nucleotide-free chaperone. Our findings of an interaction between HrHRF and EF-1delta taken with some of the recently published information concerning the TCTP (HrHRF) mentioned above suggest a possible intracellular role for TCTP/HrHRF.
人重组组胺释放因子（HrHRF），也被称为翻译控制肿瘤蛋白（TCTP）、p23和福替林，已被描述具有细胞外和细胞内功能。为了阐明HrHRF在细胞外或细胞内的作用，我们以HrHRF为诱饵，利用酵母双杂交系统和Jurkat T细胞文库。我们分离出了编码氨基酸12至281的人延伸因子-1δ（EF-1δ）的部分cDNA克隆。这种相互作用通过免疫共沉淀实验得到了证实。此前，在众多研究中，HrHRF和EF-1δ均已被分离并鉴定与恶性肿瘤相关。EF-1δ是负责蛋白质合成动力学校对的EF-1复合物的一部分。此外，DNA微阵列数据将TCTP（HrHRF）归类为与核糖体蛋白共同调控，并且最近对TCTP（HrHRF）的结构分析将其与一种无鸟嘌呤核苷酸的伴侣蛋白联系起来。我们关于HrHRF与EF-1δ之间相互作用的发现，结合上述一些最近发表的关于TCTP（HrHRF）的信息，提示TCTP/HrHRF可能具有细胞内作用。
