Romanick Mark A, Rakoczy Sharlene G, Brown-Borg Holly M
Department of Pharmacology, Physiology, and Therapeutics, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58203, USA.
Mech Ageing Dev. 2004 Apr;125(4):269-81. doi: 10.1016/j.mad.2004.02.001.
Resting and exercised (both acute and chronic) hindlimb skeletal muscle from long-lived Ames dwarf and wild type mice at 3, 12, 18, and 24 months of age was tested for antioxidant enzyme activity and protein, non-enzymatic antioxidant ratios, mitochondrial hydrogen peroxide concentration, and plasma lactate levels. Differences were observed in GPX enzyme activity between mouse genotypes at all physical activity levels, with dwarf mice exhibiting depressed levels at younger ages (3 months: P = 0.09 [non-swim], P = 0.03 [acute swim], P = 0.04 [chronic swim]) and comparatively higher levels than wild type mice at older ages (18-24 months: P = 0.05 [acute swim], P = 0.07 [chronic swim]). Catalase enzyme activity and the GSH system rarely demonstrated significant differences between genotypes, regardless of age or activity. However, the chronic exercise group displayed a difference in GSH:GSSG ratios between mouse genotypes (P = 0.005). Plasma lactate concentrations were elevated in the wild type mice compared to the dwarf mice at all ages in all activity groups. These results suggest there are biological differences with regard to antioxidant defense that favor the Ames dwarf mouse in active and resting skeletal muscle when compared to wild type mice.
对3、12、18和24月龄的长寿艾姆斯侏儒小鼠和野生型小鼠的静息及运动（急性和慢性）后肢骨骼肌进行了抗氧化酶活性和蛋白质、非酶抗氧化剂比率、线粒体过氧化氢浓度及血浆乳酸水平的检测。在所有身体活动水平下，均观察到小鼠基因型之间谷胱甘肽过氧化物酶（GPX）酶活性存在差异，侏儒小鼠在较年轻时（3个月：非游泳组P = 0.09，急性游泳组P = 0.03，慢性游泳组P = 0.04）酶活性水平较低，而在较年长时（18 - 24个月：急性游泳组P = 0.05，慢性游泳组P = 0.07）酶活性水平比野生型小鼠相对较高。无论年龄或活动情况如何，过氧化氢酶活性和谷胱甘肽（GSH）系统在基因型之间很少表现出显著差异。然而，慢性运动组在小鼠基因型之间的GSH:GSSG比率上存在差异（P = 0.005）。在所有活动组的所有年龄段，野生型小鼠的血浆乳酸浓度均高于侏儒小鼠。这些结果表明，与野生型小鼠相比，在抗氧化防御方面存在生物学差异，这有利于艾姆斯侏儒小鼠的活跃和静息骨骼肌。
