Nishimoto Ines Nobuko, Pinheiro Nidia Alice, Rogatto Silvia Regina, Carvalho André Lopes, Simpson Andrew J, Caballero Otávia Luisa, Kowalski Luiz Paulo
Department of Head and Neck Surgery and Otorhinolaryngology, Centro de Tratamento e Pesquisa Hospital do Câncer A.C. Camargo, Rua Antonio Prudente, 211, São Paulo SP-01509-900, Brazil.
Oral Oncol. 2004 Jul;40(6):604-10. doi: 10.1016/j.oraloncology.2003.12.009.
Squamous cell carcinoma of the upper aerodigestive tract (UADT) is associated with environmental factors, especially tobacco and alcohol consumption. Genetic factors, including cyclin D1 (CCND1) polymorphism have been suggested to play an important role in tumorigenesis and progression of UADT cancer. To investigate the relationship between CCND1 polymorphism on susceptibility for UADT cancers, 147 cancer and 135 non-cancer subjects were included in this study. CCND1 genotype at codon 242(G870A) in exon 4 was undertaken using denaturing high performance liquid chromatography (DHPLC) and DNA sequencing. Significant odds ratio (OR) of the AA+GA genotypes [OR=7.5 (95% CI: 1.4-39.7)] was observed in non-drinkers but for non-smokers a non-significant [OR=5.4 (95% CI: 0.9-31.4)] was found in the adjusted model. These results suggest that allele A may be a risk factor for UADT cancer, especially in non-alcoholics. However, further epidemiological studies are needed to establish the exact role of CCND1 polymorphism and the development of UADT cancers.
上呼吸消化道鳞状细胞癌（UADT）与环境因素有关，尤其是烟草和酒精消费。包括细胞周期蛋白D1（CCND1）多态性在内的遗传因素被认为在UADT癌症的发生和发展中起重要作用。为了研究CCND1多态性与UADT癌症易感性之间的关系，本研究纳入了147例癌症患者和135例非癌症患者。使用变性高效液相色谱（DHPLC）和DNA测序对第4外显子242密码子（G870A）处的CCND1基因型进行检测。在不饮酒者中观察到AA + GA基因型的显著优势比（OR）[OR = 7.5（95% CI：1.4 - 39.7）]，但在不吸烟者中，在调整模型中发现无显著差异[OR = 5.4（95% CI：0.9 - 31.4）]。这些结果表明，等位基因A可能是UADT癌症的危险因素，尤其是在不饮酒者中。然而，需要进一步的流行病学研究来确定CCND1多态性的确切作用以及UADT癌症的发生发展。
