Brayden David J, Baird Alan W
Faculty of Veterinary Medicine and Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland.
Adv Drug Deliv Rev. 2004 Apr 19;56(6):721-6. doi: 10.1016/j.addr.2003.10.036.
Apical membrane-located receptors on intestinal Peyer's patch M cells have been identified as pathogen binding sites which have potential as targets for the delivery of formulated subunit and recombinant vaccine antigens. Gene expression studies have correlated outputs from a series of models including human intestinal M-like cell cultures and human Peyer's patch tissue. Outputs comprise novel receptors with unknown function and also conserved binding sites for an ever-increasing list of pathogens. Screening for ligands to mimic pathways used by selected pathogens for invading the human intestine has the potential for increasing the efficiency of delivery of oral vaccine antigens to M cells and consequently to sub-epithelial sites for antigen processing. Synthesis of efficient and specific targeting ligands to human M cells may eventually lead to formulations based on the formats of targeted antigen-loaded nanoparticles or ligand-conjugated stable antigens.
肠道派尔集合淋巴结M细胞顶端膜上的受体已被确定为病原体结合位点，具有作为递送配方亚单位和重组疫苗抗原靶点的潜力。基因表达研究将一系列模型（包括人肠道M样细胞培养物和人派尔集合淋巴结组织）的结果进行了关联。这些结果包括功能未知的新型受体以及越来越多病原体的保守结合位点。筛选模拟特定病原体侵入人体肠道所使用途径的配体，有可能提高口服疫苗抗原向M细胞的递送效率，进而提高向抗原加工的上皮下位点的递送效率。合成针对人M细胞的高效特异性靶向配体，最终可能会形成基于靶向负载抗原的纳米颗粒或配体偶联稳定抗原形式的制剂。
