Bosso F J, Maron M B, Pilati C F, Jarjoura D G
Department of Physiology, Northeastern Ohio Universities College of Medicine, Rootstown 44272.
J Appl Physiol (1985). 1992 Jul;73(1):50-8. doi: 10.1152/jappl.1992.73.1.50.
We evaluated the ability of the canine in situ left lower lobe (LLL) vasculature to sieve endogenous plasma proteins of various molecular radii (34-124 A) after LLL arterial pressure had been transiently elevated to 23.8 +/- 0.9 (control group, n = 5) or 92.3 +/- 1.4 (SE) Torr (high-pressure group, n = 9) by restricting LLL venous outflow under conditions of constant flow. After LLL flow was returned to natural perfusion, left atrial pressure was elevated in step increments, and LLL lymph and blood samples were collected until filtration-independent lymph-to-plasma protein concentration ratios (CL/CP) were obtained. The osmotic reflection coefficients (sigma d) for total proteins and seven protein fractions (separated by gradient gel electrophoresis) were calculated. The average total protein sigma d of the high-pressure group [0.51 +/- 0.06 (SE)] was significantly lower than that of the control group (0.68 +/- 0.03). Several LLLs of the high-pressure group, however, exhibited normal sigma d's. Protein fraction CL/CP's decreased with increasing molecular radius in both groups, but the CL/CP-molecular radius relationship was displaced upward in the high-pressure group. Pore analysis suggested that the decreases in sigma d could be explained by increases in the fractional flow through a large-pore system.
我们评估了在恒定流量条件下，通过限制左肺下叶（LLL）静脉流出，使LLL动脉压短暂升高至23.8±0.9（对照组，n = 5）或92.3±1.4（标准误）托（高压组，n = 9）后，犬原位LLL脉管系统筛选不同分子半径（34 - 124 Å）内源性血浆蛋白的能力。在LLL血流恢复自然灌注后，逐步升高左心房压力，并采集LLL淋巴液和血液样本，直至获得与滤过无关的淋巴液与血浆蛋白浓度比（CL/CP）。计算了总蛋白和七个蛋白组分（通过梯度凝胶电泳分离）的渗透反射系数（σd）。高压组的平均总蛋白σd[0.51±0.06（标准误）]显著低于对照组（0.68±0.03）。然而，高压组的几个LLL表现出正常的σd值。两组中，蛋白组分的CL/CP均随分子半径增加而降低，但高压组中CL/CP与分子半径的关系向上偏移。孔隙分析表明，σd的降低可以通过大孔隙系统中分流分数的增加来解释。
