Parker R E, Roselli R J, Brigham K L
J Appl Physiol (1985). 1985 Mar;58(3):869-75. doi: 10.1152/jappl.1985.58.3.869.
Experiments were conducted in seven chronically instrumented unanesthetized sheep to estimate the osmotic reflection coefficient (sigma d) for total proteins and the solvent-drag reflection coefficients (sigma f) for six endogenous protein fractions. We measured the lymph-to-plasma ratio of total proteins (CL/CP) and six protein fractions during base-line conditions and after left atrial pressure elevations of 24-26 h per elevation. We also monitored pulmonary arterial pressure, left atrial pressure, systemic arterial pressure, and lung lymph flow at the various levels of pulmonary microvascular pressure. Our results indicate the CL/CP may require up to 24 h to reach a true steady state. It was found that sigma d is at least 0.89 for total proteins and sigma f is at least 0.84, 0.87, 0.86, 0.92, 0.95, and 0.96 for protein fractions with effective molecular radii of 36, 39.5, 44, 66, 105, and 123 A, respectively. In addition, the sigma f values for various protein fractions obtained from this investigation are compared with the predicted values of various mathematical models of the lung microcirculation.
在七只长期植入仪器且未麻醉的绵羊身上进行了实验，以估计总蛋白的渗透反射系数（σd）和六种内源性蛋白组分的溶剂拖曳反射系数（σf）。我们在基线条件下以及每次左心房压力升高24 - 26小时后，测量了总蛋白（CL/CP）和六种蛋白组分的淋巴与血浆比率。我们还监测了在不同肺微血管压力水平下的肺动脉压、左心房压、体动脉压和肺淋巴流量。我们的结果表明，CL/CP可能需要长达24小时才能达到真正的稳态。研究发现，总蛋白的σd至少为0.89，有效分子半径分别为36、39.5、44、66、105和123埃的蛋白组分的σf分别至少为0.84、0.87、0.86、0.92、0.95和0.96。此外，将本次研究获得的各种蛋白组分的σf值与肺微循环各种数学模型的预测值进行了比较。
