Nisbet Alasdair J, Gasser Robin B
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia.
Int J Parasitol. 2004 Apr;34(5):633-43. doi: 10.1016/j.ijpara.2003.12.007.
Gender-specific gene expression in Trichostrongylus vitrinus (order Strongylida) was investigated by constructing male- and female-specific gene archives using a suppressive-subtractive hybridisation approach, sequencing of expressed sequence tags from these archives, comparison with genes of Caenorhabditis elegans and other organisms, and expression profiling of a representative subset of 716 expressed sequence tags by microarray and macroarray analysis. Of these T. vitrinus expressed sequence tags, 391 had sequence homology to C. elegans genes. Of the remaining expressed sequence tags, 62 had homology to genes of other species of parasitic nematodes, and 263 expressed sequence tags had no significant homology. Expression profiling showed gender-specific expression for 561 of the 716 T. vitrinus expressed sequence tags. Male-specific protein kinases and protein phosphatases, major sperm proteins and enzymes involved in carbohydrate metabolism were abundant in the cDNA archive. Female-specific vitellogenins, heat-shock proteins and chaperonins were also highly represented. Genes involved in a number of cellular processes, such as ubiquitination and proteasome function, gene transcription, cell signalling, protein-protein interactions and chromatin assembly and function were also expressed in a gender-specific manner. The potential roles of these genes in gametogenesis, embryogenesis and reproduction in the parasitic nematode are discussed in relation to the known roles of their homologues in C. elegans.
通过使用抑制性消减杂交方法构建雄性和雌性特异性基因文库、对这些文库中的表达序列标签进行测序、与秀丽隐杆线虫和其他生物的基因进行比较以及通过微阵列和宏阵列分析对716个表达序列标签的代表性子集进行表达谱分析，研究了玻璃毛圆线虫（圆线目）中的性别特异性基因表达。在这些玻璃毛圆线虫表达序列标签中，391个与秀丽隐杆线虫基因具有序列同源性。在其余的表达序列标签中，62个与其他寄生线虫物种的基因具有同源性，263个表达序列标签没有显著同源性。表达谱分析显示，716个玻璃毛圆线虫表达序列标签中有561个具有性别特异性表达。雄性特异性蛋白激酶和蛋白磷酸酶、主要精子蛋白以及参与碳水化合物代谢的酶在cDNA文库中丰富。雌性特异性卵黄蛋白原、热休克蛋白和伴侣蛋白也高度富集。参与许多细胞过程的基因，如泛素化和蛋白酶体功能、基因转录、细胞信号传导、蛋白质-蛋白质相互作用以及染色质组装和功能，也以性别特异性方式表达。结合其同源物在秀丽隐杆线虫中的已知作用，讨论了这些基因在寄生线虫配子发生、胚胎发生和繁殖中的潜在作用。
