Delaval Bénédicte, Ferrand Alexia, Conte Nathalie, Larroque Christian, Hernandez-Verdun Danièle, Prigent Claude, Birnbaum Daniel
Department of Molecular Oncology, U119 Inserm, Institut Paoli-Calmettes, IFR57, Marseille, France.
Oncogene. 2004 Jun 3;23(26):4516-22. doi: 10.1038/sj.onc.1207593.
Taxins are a family of centrosomal proteins important for the regulation of mitosis and microtubule dynamics. Cytokinesis, the last step of M phase, is essential for chromosomal integrity and cell division. It is highly regulated and involves a reorganization of microtubules and actin filaments. We show here that TACC1 localizes diffusely to the midzone spindle in anaphase and strongly to the midbody during cytokinesis, indicating a possible involvement of this protein in the exit of M phase. TACC1 also relocalizes to the nucleolus in interphase. We demonstrate that TACC1 and the mitotic kinase Aurora B belong to the same complex during cytokinesis. We further show that Aurora B knocked down by RNA-mediated interference prevents the formation of the midbody - and consequently affects TACC1 localization at this site - and leads to abnormal cell division and multinucleated cells.
Taxins是一类中心体蛋白家族，对有丝分裂和微管动力学的调节很重要。胞质分裂是M期的最后一步，对染色体完整性和细胞分裂至关重要。它受到高度调控，涉及微管和肌动蛋白丝的重组。我们在此表明，TACC1在后期扩散定位于纺锤体中区，在胞质分裂期间强烈定位于中间体，表明该蛋白可能参与M期的退出。TACC1在间期也重新定位于核仁。我们证明，在胞质分裂期间，TACC1和有丝分裂激酶Aurora B属于同一复合体。我们进一步表明，通过RNA介导的干扰敲低Aurora B会阻止中间体的形成——从而影响TACC1在此位点的定位——并导致异常细胞分裂和多核细胞。
