Blake-Haskins J C, Mellberg J R, Snyder C
Colgate Palmolive Company, Technology Center, Oral Research and Development Division, Piscataway, New Jersey 08854.
J Dent Res. 1992 Aug;71(8):1482-6. doi: 10.1177/00220345920710080401.
The uptake of calcium by a polysaccharide (agarose) gel used as a model for plaque from a two-step treatment (consisting of a calcium rinse followed by a fluoride treatment) and the effect of the deposited calcium in model plaque on caries lesion formation in enamel were determined. Calcium uptake was measured by treatment of the model plaques with [45Ca]-CaCl2 solutions with or without NaF. A two-step treatment consisting of calcium followed by fluoride produced a 100% increase in calcium content of model plaque, presumably due to the formation of CaF2, compared with a treatment with artificial saliva followed by calcium alone. The effects of these increased plaque minerals on caries lesion formation were studied by subjecting model-plaque-covered enamel blocks to a cyclic demineralization-remineralization treatment. Artificial-plaque-covered enamel blocks were treated daily with 180 ppm calcium for ten min, then 100 ppm fluoride for ten min, followed by demineralization for 16 h, and finally, remineralization for seven h and 40 min. After five days, the blocks were sectioned, and lesion formation was determined by microradiography-microdensitometry. Artificial plaque treated with a calcium rinse followed by a fluoride rinse reduced lesion size by 90%, compared with a 68% reduction by a fluoride rinse alone. When the experiment was repeated with a simulated pre-brush calcium rinse (180 ppm calcium) followed by a fluoride dentifrice suspension (110 ppm fluoride), lesion size was reduced by 46%, compared with a 32% reduction by the fluoride dentifrice suspension alone.
研究了一种用作牙菌斑模型的多糖（琼脂糖）凝胶对两步处理（先进行钙冲洗，然后进行氟处理）中钙的摄取情况，以及模型牙菌斑中沉积的钙对牙釉质龋损形成的影响。通过用含或不含氟化钠的[45Ca]-氯化钙溶液处理模型牙菌斑来测量钙摄取量。与仅用人工唾液然后单独用钙处理相比，先进行钙处理再进行氟处理的两步处理使模型牙菌斑中的钙含量增加了100%，这可能是由于形成了氟化钙。通过对覆盖有模型牙菌斑的牙釉质块进行循环脱矿质-再矿质化处理，研究了这些增加的牙菌斑矿物质对龋损形成的影响。每天用180 ppm钙处理覆盖有人工牙菌斑的牙釉质块10分钟，然后用100 ppm氟处理10分钟，接着进行16小时的脱矿质处理，最后进行7小时40分钟的再矿质化处理。五天后，将这些块切片，并通过显微放射照相-显微密度测定法确定龋损形成情况。与仅用氟冲洗使病变大小减少68%相比，先用钙冲洗然后用氟冲洗处理的人工牙菌斑使病变大小减少了90%。当用模拟刷牙前的钙冲洗（180 ppm钙）然后用含氟牙膏悬浮液（110 ppm氟）重复该实验时，病变大小减少了46%，而仅用含氟牙膏悬浮液使病变大小减少了32%。
