Dusková J, Dusek J
Katedra farmaceutické botaniky a ekologie Farmaceutické fakulty Univerzity Karlovy, Hradec Králové.
Ceska Slov Farm. 2004 Jan;53(1):39-41.
Segments of sterile germinating plants were used to derive the tissue culture. Tests of the optimal hormonal composition of the medium from the standpoint of the growth of cultures revealed that the highest growth in biomass was achieved in the medium with an addition of 2.4-D (0.1, 1.0 mg.l-1), NAA (10.0 mg.l-1), and a combination of IAA (1.0 mg.l-1) + K (1.0 mg.l-1). The difference between these values was statistically insignificant. Other tested concentrations of growth regulators showed statistically significantly lower increases. The effects of different types of growth regulators and their concentrations on the production of selected metabolites in the tissue cultures were also examined and compared with the results obtained from the extract of the drug. Of alkaloids, stachydrine was demonstrated both in the extract of the drug and the extracts from all tested calluses regardless of the type and concentration of the growth regulator. Of flavonoid substances, two stains were demonstrated in the extract from the drug as well as in the extracts from all tested calluses. Neither of them was identical with the employed standards of rutin and quercetin. The results of TLC of iridoid substances were different in the extract from the drug (12 stains) and in the extracts from calluses (4 stains--3 identical with the stains in the extract from the drug). As to saccharides, three of them--glucose, fructose, and sucrose--were identified in the extract of the drug as well as in the extracts from all calluses.
使用无菌发芽植物的切段来获得组织培养物。从培养物生长的角度对培养基的最佳激素组成进行测试，结果表明，添加2.4 - D（0.1、1.0 mg.l-1）、NAA（10.0 mg.l-1）以及IAA（1.0 mg.l-1）+ K（1.0 mg.l-1）组合的培养基中生物量增长最高。这些值之间的差异在统计学上不显著。其他测试的生长调节剂浓度在统计学上显示出显著更低的增长。还研究了不同类型的生长调节剂及其浓度对组织培养物中选定代谢产物产生的影响，并与从药物提取物中获得的结果进行比较。在生物碱方面，无论生长调节剂的类型和浓度如何，在药物提取物以及所有测试愈伤组织的提取物中均检测到水苏碱。在黄酮类物质方面，在药物提取物以及所有测试愈伤组织的提取物中均检测到两种色斑。它们都与所使用的芦丁和槲皮素标准品不同。环烯醚萜类物质的薄层色谱结果在药物提取物（12个色斑）和愈伤组织提取物（4个色斑 - 其中3个与药物提取物中的色斑相同）中有所不同。至于糖类，在药物提取物以及所有愈伤组织提取物中均鉴定出其中三种 - 葡萄糖、果糖和蔗糖。
