聚（3-羟基丁酸酯-co-3-羟基己酸酯）与骨髓间充质干细胞的生物相容性
[Biocompatibility of p(3HB-co-3HH) and marrow mesenchymal stem cells].
作者信息
Zhao Yu, Hu Ping, Lu Ying-ling
机构信息
Department of Plastic Surgery, First Affiliated Hospital of Anhui University of Medical Science, Hefei, Anhui, P. R. China 230022.
出版信息
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2004 Mar;18(2):104-7.
OBJECTIVE
To investigate the biocompatibility of p(3HB-co-3HH) and marrow mesenchymal stell cells (MSCs).
METHODS
MSCs were inoculated to p(3HB-co-3HH), and then cultured for 2-4 weeks in vitro and embedded for 2 weeks in vivo. The growth, proliferation, morphology and phenotype properties of MSCs were observed by use of phase contrast microscope, electron microscope, HE staining and staining of type I collagen.
RESULTS
p(3HB-co-3HH) had good compatibility. The inoculated MSCs could be well-distributed, attached well and obtain the phenotype of MSCs in p(3HB-co-3HH). After osteogenic inducer were added, MSCs differentiated to osteoblasts and secreted matrix. Type I collagen was stained positively by immunohistochemical techenique.
CONCLUSION
The above results demonstrate that there is satisfactory biocompatibility between p(3HB-co-3HH) and MSCs.
目的
研究聚（3-羟基丁酸酯-co-3-羟基己酸酯）（p(3HB-co-3HH)）与骨髓间充质干细胞（MSCs）的生物相容性。
方法
将MSCs接种于p(3HB-co-3HH)上，然后在体外培养2-4周，并在体内包埋2周。通过相差显微镜、电子显微镜、HE染色和I型胶原染色观察MSCs的生长、增殖、形态和表型特性。
结果
p(3HB-co-3HH)具有良好的相容性。接种的MSCs能良好分布、附着，并在p(3HB-co-3HH)中获得MSCs的表型。添加成骨诱导剂后，MSCs分化为成骨细胞并分泌基质。免疫组化技术显示I型胶原呈阳性染色。
结论
上述结果表明p(3HB-co-3HH)与MSCs之间存在令人满意的生物相容性。
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