Dodi A, Monnier V
Département d'Etudes des Déchets, Direction de l'Energie Nucléaire, C.E.A. Cadarache, 13108 Saint Paul lez Durance, France.
J Chromatogr A. 2004 Apr 2;1032(1-2):87-92. doi: 10.1016/j.chroma.2003.10.095.
Ethylenediaminetetraacetic acid (EDTA) is a metal complexing agent which is commonly used for removing metallic surface contaminations. But its presence in aqueous industrial effluents or in wastes, by example those which are generated from nuclear power facilities, is forbidden or strictly regulated by the legislation. The implementation of high-performance liquid chromatography coupled with mass spectrometry (MS) via an electrospray interface has exhibited a powerful capacity in the measurement of this complexing agent, in the form of its iron complex. The complex is eluted through a reversed stationary phase under slightly acidic conditions and the detection is performed by mass spectrometry in the single ion monitoring mode. This technique has allowed us to reach a detection limit of about 1 microg/l in EDTA for only 20 microl injected without any previous preconcentration, and to solve some analytical interferences observed in the case of industrial effluents with other analytical techniques such as ion chromatography.
乙二胺四乙酸（EDTA）是一种金属络合剂，常用于去除金属表面污染物。但在工业废水或废物中，例如由核电设施产生的废水或废物中，其存在是被法律禁止或严格监管的。通过电喷雾接口将高效液相色谱与质谱（MS）联用，已显示出在测定这种以铁络合物形式存在的络合剂方面具有强大的能力。该络合物在微酸性条件下通过反相固定相洗脱，并通过质谱在单离子监测模式下进行检测。这项技术使我们在仅注入20微升且无需任何预先富集的情况下，就能达到约1微克/升的EDTA检测限，并解决了在工业废水分析中使用其他分析技术（如离子色谱）时观察到的一些分析干扰问题。
