Angelico M, Ginanni Corradini S, Masella R, Alvaro D, Cantafora A, Capocaccia L
2nd Gastroenterology Division, University of Rome La Sapienza, School of Medicine, Italy.
J Hepatol. 1992 May;15(1-2):59-66. doi: 10.1016/0168-8278(92)90012-e.
It has been suggested that qualitative changes in bile phosphatidylcholine (lecithin) play a role in the pathogenesis of cholesterol gallstones. We investigated the possible relationship between the molecular composition and hydrophobicity of biliary lecithins and bile cholesterol saturation, nucleation time and the mode of cholesterol transport in human gallbladder bile. Nineteen patients (12 with and seven without gallstones) undergoing abdominal surgery were studied. Bile cholesterol saturation ranged from 77% to 186% (median: 123%) and nucleation time from 1 to 24 days (median: 3 days). Biliary lipid carriers (vesicles and mixed micelles) were separated using Superose-6 gel chromatography and their lipid content was quantitated. Biliary lecithin composition was analyzed by HPLC. Fourteen individual molecular species were detected in the bile: none were related to cholesterol saturation or nucleation time. An arbitrary cumulative index of lecithin hydrophobicity was computed for each bile sample, based on the HPLC capacity factor of the individual species and their percent mole fraction: this index ranged from 47.0 and 58.5 (median: 49) and was unrelated to cholesterol saturation and nucleation time. The biliary concentration of sn-1 palmitoyl:sn-2 arachidonoyl lecithin was significantly correlated (p less than 0.01) with the fraction of cholesterol carried by mixed micelles. This finding suggests that arachidonoyl lecithin may play a modulatory role in the partitioning of cholesterol among biliary carriers. We conclude that major abnormalities in the composition of biliary lecithins are unlikely to play a causative role in the pathogenesis of cholesterol gallstone, although the role of arachidonoyl species requires further investigation.
有人提出，胆汁磷脂酰胆碱（卵磷脂）的质的变化在胆固醇性胆结石的发病机制中起作用。我们研究了胆汁卵磷脂的分子组成和疏水性与胆汁胆固醇饱和度、成核时间以及人胆囊胆汁中胆固醇转运模式之间的可能关系。对19例接受腹部手术的患者（12例有胆结石，7例无胆结石）进行了研究。胆汁胆固醇饱和度范围为77%至186%（中位数：123%），成核时间为1至24天（中位数：3天）。使用Superose - 6凝胶色谱法分离胆汁脂质载体（囊泡和混合微团），并对其脂质含量进行定量。通过高效液相色谱法分析胆汁卵磷脂组成。在胆汁中检测到14种单个分子种类：均与胆固醇饱和度或成核时间无关。基于单个种类的高效液相色谱容量因子及其摩尔分数百分比，为每个胆汁样本计算了一个任意的卵磷脂疏水性累积指数：该指数范围为47.0至58.5（中位数：49），与胆固醇饱和度和成核时间无关。sn - 1棕榈酰：sn - 2花生四烯酰卵磷脂的胆汁浓度与混合微团携带的胆固醇分数显著相关（p小于0.01）。这一发现表明花生四烯酰卵磷脂可能在胆固醇在胆汁载体之间的分配中起调节作用。我们得出结论，胆汁卵磷脂组成的主要异常不太可能在胆固醇性胆结石的发病机制中起致病作用，尽管花生四烯酰种类的作用需要进一步研究。
