蜡样芽孢杆菌ATCC 14579在特定条件下的生长与芽孢形成：关键σ因子基因的时序表达
Growth and sporulation of Bacillus cereus ATCC 14579 under defined conditions: temporal expression of genes for key sigma factors.
作者信息
de Vries Ynte P, Hornstra Luc M, de Vos Willem M, Abee Tjakko
机构信息
Wageningen Centre for Food Sciences, Laboratory of Food Microbiology, Wageningen UR, The Netherlands.
出版信息
Appl Environ Microbiol. 2004 Apr;70(4):2514-9. doi: 10.1128/AEM.70.4.2514-2519.2004.
Abstract
An airlift fermentor system allowing precise regulation of pH and aeration combined with a chemically defined medium was used to study growth and sporulation of Bacillus cereus ATCC 14579. Sporulation was complete and synchronous. Expression of sigA, sigB, sigF, and sigG was monitored with real-time reverse transcription-PCR, and the pattern qualitatively resembled that of Bacillus subtilis. This method allows reproducible production of stable spores, while the synchronous growth and defined conditions are excellently suitable for further gene expression studies of cellular differentiation of B. cereus.
摘要
使用一种能够精确调节pH值和通气量并结合化学成分明确培养基的气升式发酵罐系统，来研究蜡样芽孢杆菌ATCC 14579的生长和孢子形成。孢子形成完整且同步。通过实时逆转录PCR监测sigA、sigB、sigF和sigG的表达，其模式在质量上类似于枯草芽孢杆菌。该方法能够可重复地生产稳定的孢子，同时同步生长和明确的条件非常适合进一步开展蜡样芽孢杆菌细胞分化的基因表达研究。
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