Swammerdam Institute for Life Sciences, Department of Molecular Biology and Microbial Food Safety, University of Amsterdam, 1098 XH Amsterdam, The Netherlands.
Department of Medical Microbiology, Centre for Infection and Immunity Amsterdam (CINIMA), Academic Medical Centre, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands.
Int J Mol Sci. 2020 Nov 27;21(23):8967. doi: 10.3390/ijms21238967.
The occurrence of bacterial pathogens in the food chain has caused a severe impact on public health and welfare in both developing and developed countries. Moreover, the existence of antimicrobial-tolerant persisting morphotypes of these pathogens including both persister-cells as well as bacterial spores contributes to difficulty in elimination and in recurrent infection. Therefore, comprehensive understanding of the behavior of these persisting bacterial forms in their environmental niche and upon infection of humans is necessary. Since traditional antimicrobials fail to kill persisters and spores due to their (extremely) low metabolic activities, antimicrobial peptides (AMPs) have been intensively investigated as one of the most promising strategies against these persisting bacterial forms, showing high efficacy of inactivation. In addition, AMP-based foodborne pathogen detection and prevention of infection has made significant progress. This review focuses on recent research on common bacterial pathogens in the food chain, their persisting morphotypes, and on AMP-based solutions. Challenges in research and application of AMPs are described.
食源致病菌的出现对发展中国家和发达国家的公共卫生和福利造成了严重影响。此外，这些致病菌具有抗药性的持久形态，包括休眠细胞和细菌孢子，这使得它们难以消除，并且容易再次感染。因此，全面了解这些持久细菌形态在其环境生态位中的行为以及在人类感染时的行为是必要的。由于传统的抗菌药物由于其（极）低的代谢活性而无法杀死休眠细胞和孢子，因此抗菌肽（AMPs）已被作为最有前途的策略之一进行了深入研究，以对抗这些持久的细菌形态，显示出高效的灭活作用。此外，基于 AMP 的食源性病原体检测和感染预防也取得了重大进展。本综述重点介绍了食源致病菌及其持久形态的最新研究进展，以及基于 AMP 的解决方案。描述了 AMP 研究和应用中的挑战。
