Sandrin M S, Gumley T P, Henning M M, Vaughan H A, Gonez L J, Trapani J A, McKenzie I F
Molecular Immunogenetics Laboratory, Austin Research Institute, Austin Hospital Heidelberg, Victoria, Australia.
J Immunol. 1992 Sep 1;149(5):1636-41.
We describe the production and characterization of a mouse mAb, S-450-33.2, recognizing the Ly-9.2 specificity. This mAb was used to purify Ly-9 molecules from lymphoid cell lines, and the amino-terminal amino acids were determined. The mAb was also used in a eukaryotic expression system, to isolate cDNA clones encoding Ly-9. Analysis of RNA showed that Ly-9 expression is lymphocyte specific, as determined by the presence of a single hybridizing 2.4-kb species found only in lymphoid cells. Genomic DNA analysis indicated that Ly-9 is encoded by a single-copy gene of 10 to 15 kb. The predicted polypeptide belongs to the Ig superfamily of cell surface molecules with four extracellular Ig-like domains, i.e., a non-disulfide-bonded V domain, a truncated C2 domain with two disulfide bonds, a second non-disulfide-bonded V domain, and a truncated C2 domain with two disulfide bonds (V-C2-V-C2). The sequence data also support the view that Ly-9 belongs to the subgroup of the Ig superfamily that includes Bcm-1, CD2, and LFA-3.
我们描述了一种识别Ly-9特异性的小鼠单克隆抗体S-450-33.2的制备及其特性。该单克隆抗体用于从淋巴细胞系中纯化Ly-9分子，并确定了其氨基末端氨基酸。该单克隆抗体还用于真核表达系统，以分离编码Ly-9的cDNA克隆。RNA分析表明，Ly-9表达具有淋巴细胞特异性，这是通过仅在淋巴细胞中发现的单一杂交2.4-kb条带确定的。基因组DNA分析表明，Ly-9由一个10至15 kb的单拷贝基因编码。预测的多肽属于细胞表面分子的免疫球蛋白超家族，具有四个细胞外免疫球蛋白样结构域，即一个非二硫键连接的V结构域、一个具有两个二硫键的截短C2结构域、第二个非二硫键连接的V结构域和一个具有两个二硫键的截短C2结构域（V-C2-V-C2）。序列数据也支持Ly-9属于免疫球蛋白超家族中包括Bcm-1、CD2和LFA-3的亚组的观点。
