Sandrin M S, Henning M M, Lo M F, Baker E, Sutherland G R, McKenzie I F
Molecular Immunogenetics Laboratory, Austin Research Institute, Austin Hospital, Heidelberg, Vic, Australia.
Immunogenetics. 1996;43(1-2):13-9. doi: 10.1007/BF00186599.
Ly9 is a mouse cell membrane antigen found on all lymphocytes and coded for by a gene that maps to chromosome 1. We previously described the isolation and characterization of a full-length cDNA clone for mouse Ly9. Using cross-species hybridization we isolated cDNA clones encoding the human homologue Humly9. Analysis of the predicted protein sequence suggests that the extra-cellular portion of the Humly9 molecules is composed of four Ig-like domains: a V domain (V) without disulphide bonds and a truncated C2 domain (tC2) with two disulphide bonds, a second V domain without disulphide bonds and a second tC2 with two disulphide bonds, i.e., as V-tC2-V-tC2. The gene encoding Humly9 was mapped to chromosome 1 by analysis of human/hamster hybrids, and more specifically to the 1q22 region by in situ hybridization. The protein sequence data support the view that Humly9 belongs to the immunoglobulin-superfamily subgroup which includes CD48, CD2, and LFA-3.
Ly9是一种在所有淋巴细胞上发现的小鼠细胞膜抗原，由一个定位于1号染色体的基因编码。我们之前描述了小鼠Ly9全长cDNA克隆的分离和特性。利用种间杂交，我们分离出了编码人同源物Humly9的cDNA克隆。对预测的蛋白质序列分析表明，Humly9分子的细胞外部分由四个免疫球蛋白样结构域组成：一个没有二硫键的V结构域（V）和一个有两个二硫键的截短C2结构域（tC2），第二个没有二硫键的V结构域和第二个有两个二硫键的tC2结构域，即V-tC2-V-tC2。通过对人/仓鼠杂种细胞的分析，编码Humly9的基因被定位于1号染色体，更具体地说，通过原位杂交定位于1q22区域。蛋白质序列数据支持Humly9属于免疫球蛋白超家族亚组的观点，该亚组包括CD48、CD2和LFA-3。
