Fogt F, Zimmerman R L, Daly T, Gausas R E
Pathology and Laboratory Medicine, University of Pennsylvania, Presbyterian Medical Center, Philadelphia, PA 19104, USA.
Int J Mol Med. 2004 May;13(5):681-3. doi: 10.3892/ijmm.13.5.681.
In the absence of antibodies specific for lymphatic vessels, analysis of lymphatic vessels within different tissues has been widely performed with light microscopic and, most importantly, electron microscopic techniques. In regard to lymphatic vessels in the ocular globe and the periocular structures, controversy remains about the specific distribution of lymphatic channels. It is postulated that bulbar and retrobulbar tissues are devoid of lymphatic vessels, but lymphatic vessels have been demonstrated in lacrimal gland and epibulbar conjunctiva. In this study, we analyzed orbital fat for the presence of lymphatic tissue using D2-40, a monoclonal antibody, specific for lymphatic vessels. We found lymphatic vessels present within bulbar conjunctiva extending to the level of the ciliary apparatus. No lymphatics were identified in healthy anterior orbital adipose tissue. In two cases of orbital mucor-mycosis and one case of panendophthalmitis, significant lymphovascular proliferation was present within granulation tissue associated with the acute inflammation. We conclude that lymph vessel proliferation may be induced in inflammatory conditions in tissues which are normally devoid of lymph channels.
在缺乏针对淋巴管的特异性抗体的情况下，利用光学显微镜技术，尤其是电子显微镜技术，已广泛开展了对不同组织内淋巴管的分析。关于眼球及眼周结构中的淋巴管，淋巴管的具体分布仍存在争议。据推测，眼球和球后组织没有淋巴管，但泪腺和球结膜已证实存在淋巴管。在本研究中，我们使用一种针对淋巴管的单克隆抗体D2-40分析眼眶脂肪中是否存在淋巴组织。我们发现球结膜内存在淋巴管，并延伸至睫状体水平。在健康的眶前脂肪组织中未发现淋巴管。在两例眼眶毛霉菌病和一例全眼球炎中，与急性炎症相关的肉芽组织内出现了显著的淋巴管增生。我们得出结论，在正常情况下没有淋巴通道的组织中，炎症状态可能会诱导淋巴管增生。
