Li Lai-Sheng, Da Shi-Lu, Feng Yu-Qi, Liu Min
Department of Chemistry, Wuhan University, Wuhan 430072, P R China.
Anal Sci. 2004 Mar;20(3):561-4. doi: 10.2116/analsci.20.561.
A new p-tert-butyl-calix[8]arene-bonded silica gel stationary phase (CABS) was prepared via 3-glycidoxypropyltrimethoxysilane as a coupling reagent for HPLC. Its structure was characterized by diffuse reflectance infrared Fourier transform spectroscopy (DRIFT), elemental analysis and thermal analysis. The chromatographic performance of new packing was evaluated by using basic, acidic and neutral aromatic compounds as probes compared with conventional ODS. The results show that the new stationary phase has an excellent reversed-phase property and high selectivities for substituted aromatics compared with ODS, because CABS can provide various sites for the analytes, such as hydrogen-bonding interactions, pi-pi interactions, and inclusion complex, besides hydrophobic interactions.
通过3-缩水甘油氧基丙基三甲氧基硅烷作为偶联剂制备了一种用于高效液相色谱的新型对叔丁基杯[8]芳烃键合硅胶固定相（CABS）。采用漫反射红外傅里叶变换光谱（DRIFT）、元素分析和热分析对其结构进行了表征。以碱性、酸性和中性芳香族化合物为探针，与传统的十八烷基硅烷键合硅胶（ODS）相比，对新型填料的色谱性能进行了评价。结果表明，与ODS相比，新型固定相具有优异的反相性能和对取代芳烃的高选择性，因为CABS除了疏水相互作用外，还能为分析物提供各种作用位点，如氢键相互作用、π-π相互作用和包合物。
