Department of Chemistry, Wuhan University, 430072 Wuhan, China; College of Chemistry and Material Sciences, Nanchang University, 330047 Nanchang, China.
Talanta. 2004 Feb 27;62(3):643-8. doi: 10.1016/j.talanta.2003.09.015.
A new p-tert-butyl-calix[8]arene-bonded silica gel stationary phase (CABS) was used for the separation of some aromatic carboxylic acids by HPLC. The chromatographic behaviour of the solutes on CABS was studied in comparison with conventional ODS. The results show that the calix[8]arene-bonded phase exhibits a stronger retention and better selectivity than ODS for the aromatic carboxylic acids. The different elution order of the analytes was also observed on both packings, which show the existence of distinct retention mechanisms. According to chromatographic data, it can be concluded that the high selectivity of CABS for aromatic carboxylic acids ascribes to various interactions between CABS and the analytes, such as hydrophobic interaction, hydrogen bonding interaction, pi-pi interaction and inclusion interaction.
一种新型的 p-叔丁基杯[8]芳烃键合硅胶固定相（CABS）被用于高效液相色谱法分离一些芳香族羧酸。将溶质在 CABS 上的色谱行为与传统的 ODS 进行了比较研究。结果表明，与 ODS 相比，杯[8]芳烃键合相对于芳香族羧酸具有更强的保留和更好的选择性。在这两种固定相上也观察到了分析物的不同洗脱顺序，这表明存在不同的保留机制。根据色谱数据，可以得出结论，CABS 对芳香族羧酸具有高选择性归因于 CABS 和分析物之间的各种相互作用，如疏水相互作用、氢键相互作用、π-π相互作用和包合相互作用。
