Di Gregorio Simona, Zocca Chiara, Sidler Stephan, Toffanin Annita, Lizzari Daniela, Vallini Giovanni
Department of Science and Technology, Laboratories of Microbial Biotechnology and Environmental Microbiology, University of Verona, Strada Le Grazie 15-Ca' Vignal, 1-37134 Verona, Italy.
Biodegradation. 2004 Apr;15(2):111-23. doi: 10.1023/b:biod.0000015624.52954.b6.
A novel genotype for the initial steps of the oxidative degradation of dibenzothiophene (DBT) is described in a Burkholderia sp. strain isolated from a drain receiving oil refinery wastewater. The strain is capable of transforming DBT with significant efficiency when compared to other microorganisms. Its genotype was discovered by investigating insertional mutants of genes involved in DBT degradation by the Kodama pathway. The cloned dbt genes show a novel genomic organization when compared to previously described genes capable of DBT catabolism in that they constitute two distinct operons and are not clustered in a single transcript. Sequence analysis suggests the presence of a sigma54-dependent positive transcriptional regulator that may be involved in the control of the transcription of the two operons, both activated by DBT. The achieved results suggest the possibility of novel features of DBT biotransformation in nature.
从接收炼油厂废水的排水管道中分离出的一株伯克霍尔德氏菌（Burkholderia sp.）中，描述了一种用于二苯并噻吩（DBT）氧化降解初始步骤的新型基因型。与其他微生物相比，该菌株能够高效转化DBT。通过研究参与通过Kodama途径降解DBT的基因的插入突变体，发现了其基因型。与先前描述的能够进行DBT分解代谢的基因相比，克隆的dbt基因显示出一种新型的基因组组织，因为它们构成了两个不同的操纵子，并且没有聚集在单个转录本中。序列分析表明存在一种可能参与控制两个操纵子转录的σ54依赖性正转录调节因子，这两个操纵子均由DBT激活。所取得的结果表明自然界中DBT生物转化可能具有新特性。
