Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy.
J Basic Microbiol. 2014 May;54(5):464-9. doi: 10.1002/jobm.201200408. Epub 2013 May 17.
Burkholderia fungorum DBT1, first isolated from settling particulate matter of an oil refinery wastewater, is a bacterial strain which has been shown capable of utilizing several polycyclic aromatic hydrocarbons (PAHs) including dibenzothiophene (DBT). In particular, this microbe is able to efficiently degrade DBT through the Kodama pathway. Previous investigations have lead to the identification of six genes, on a total of eight, required for DBT degradation. In the present study, a combined experimental/computational approach was adopted to identify and in silico characterize the two missing genes, namely a ferredoxin reductase and a hydratase-aldolase. Thus, the finding of all enzymatic components of the Kodama pathway in B. fungorum DBT1 makes this bacterial strain amenable for possible exploitation in soil bioremediation protocols.
从炼油厂废水沉降颗粒物中首次分离到的真菌伯克霍尔德氏菌 DBT1 是一种能够利用多种多环芳烃（PAHs）包括二苯并噻吩（DBT）的细菌菌株。特别是，这种微生物能够通过 Kodama 途径有效地降解 DBT。先前的研究已经确定了总共 8 个基因中的 6 个基因是 DBT 降解所必需的。在本研究中，采用了组合实验/计算方法来鉴定和计算机理表征缺失的两个基因，即一种铁氧还蛋白还原酶和一种水合酶-醛缩酶。因此，在真菌伯克霍尔德氏菌 DBT1 中发现了 Kodama 途径的所有酶促成分，这使得该细菌菌株适合于可能在土壤生物修复方案中利用。
