Shao H J, Chen L, Shen M S, Yu G F
The Key Laboratory of Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen, 361005, PR China.
Acta Virol. 2003;47(4):217-21.
DNA vaccines have been widely used as effective means of eradicating a variety of viruses, parasites, bacteria as well as means of alleviating allergic and autoimmune diseases and tumors. As interleukin 1 (IL-1) plays an essential role in augmenting both cellular and humoral immune responses to foreign antigens, it may represent a good candidate for an adjuvant to DNA vaccines. Since the inflammatory activity of IL-1 may have a restricted application to DNA vaccines, we explored the possibility of augmenting immune response without unwanted inflammatory effect using IL-1beta 163-171 peptide, which is essential for IL-1 receptor 1 binding. A DNA fragment encoding the human IL-1beta 163-171 peptide of concern was fused to the Hepatitis B virus (HBV) core DNA vaccine, and injected into mice to analyze its immune responses. Compared with the control mice which received hepatitis B virus core antigen (HBcAg) alone, significant increase in not only the HBcAg-specific antibody response but also in T cell proliferation was observed in mice which received IL-1beta 163-171-HBcAg. These results suggest that the DNA fragment encoding the IL-1beta polypeptide of aa 163-171 might represent a good candidate for an adjuvant of DNA vaccines.
DNA疫苗已被广泛用作根除多种病毒、寄生虫、细菌的有效手段，以及缓解过敏性疾病、自身免疫性疾病和肿瘤的手段。由于白细胞介素1（IL-1）在增强针对外来抗原的细胞免疫和体液免疫反应中起着至关重要的作用，它可能是DNA疫苗佐剂的一个良好候选者。鉴于IL-1的炎症活性可能对DNA疫苗的应用有限制，我们探索了使用对IL-1受体1结合至关重要的IL-1β 163-171肽来增强免疫反应而不产生不良炎症效应的可能性。将编码人IL-1β 163-171肽的DNA片段与乙肝病毒（HBV）核心DNA疫苗融合，并注射到小鼠体内以分析其免疫反应。与仅接受乙肝病毒核心抗原（HBcAg）的对照小鼠相比，接受IL-1β 163-171-HBcAg的小鼠不仅HBcAg特异性抗体反应显著增加，而且T细胞增殖也显著增加。这些结果表明，编码aa 163-171的IL-1β多肽的DNA片段可能是DNA疫苗佐剂的一个良好候选者。
