Comparative analysis using a mouse model of the immunogenicity of artificial VLP and attenuated Salmonella strain carrying a DNA-vaccine encoding HIV-1 polyepitope CTL-immunogen.

作者信息

Karpenko Larisa I, Nekrasova Nadezhda A, Ilyichev Alexander A, Lebedev Leonid R, Ignatyev George M, Agafonov Alexander P, Zaitsev Boris N, Belavin Pavel A, Seregin Sergei V, Danilyuk Nadezhda K, Babkina Irina N, Bazhan Sergei I

机构信息

The Joint-Stock Company Vector Best, The State Research Center of Virology and Biotechnology VECTOR, Novosibirsk Region, 630559 Koltsovo, Russia.

出版信息

Vaccine. 2004 Apr 16;22(13-14):1692-9. doi: 10.1016/j.vaccine.2003.09.050.

Two systems have been examined for delivery of DNA-vaccine encoding a HIV-1 polyepitope CTL-immunogen (TCI). One is intended for i.m. injection and is in the form of an artificial virus like particle containing eukaryotic expression plasmid pcDNA-TCI encapsulated within a spermidine-polyglucin conjugate. The other is intended for mucosal immunization and is based on attenuated Salmonella typhimurium strain 7207, which can deliver pcDNA-TCI directly into professional antigen-presenting cells (APC). After immunization, the artificial VLP and recombinant Salmonella induced an enhanced HIV specific serum antibody, proliferative and CTL responses compared to those induced by naked pcDNA-TCI. The most significant responses were produced when pcDNA-TCI was delivered by Salmonella.