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减毒鼠伤寒沙门氏菌递送编码鸭肝炎病毒 UL24 的 DNA 疫苗诱导全身和黏膜免疫应答，并提供良好的保护力以抵抗攻毒。

Attenuated Salmonella typhimurium delivering DNA vaccine encoding duck enteritis virus UL24 induced systemic and mucosal immune responses and conferred good protection against challenge.

机构信息

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, People's Republic of China.

出版信息

Vet Res. 2012 Jul 6;43(1):56. doi: 10.1186/1297-9716-43-56.

DOI:10.1186/1297-9716-43-56
PMID:22770566
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3412168/
Abstract

Orally delivered DNA vaccines against duck enteritis virus (DEV) were developed using live attenuated Salmonella typhimurium (SL7207) as a carrier and Escherichia coli heat labile enterotoxin B subunit (LTB) as a mucosal adjuvant. DNA vaccine plasmids pVAX-UL24 and pVAX-LTB-UL24 were constructed and transformed into attenuated Salmonella typhimurium SL7207 resulting SL7207 (pVAX-UL24) and SL7207 (pVAX-LTB-UL24) respectively. After ducklings were orally inoculated with SL7207 (pVAX-UL24) or SL7207 (pVAX-LTB-UL24), the anti-DEV mucosal and systemic immune responses were recorded. To identify the optimum dose that confers maximum protection, we used different doses of the candidate vaccine SL7207 (pVAX-LTB-UL24) during oral immunization. The strongest mucosal and systemic immune responses developed in the SL7207 (pVAX-LTB-UL24) (1011 CFU) immunized group. Accordingly, oral immunization of ducklings with SL7207 (pVAX-LTB-UL24) showed superior efficacy of protection (60-80%) against a lethal DEV challenge (1000 LD50), compared with the limited survival rate (40%) of ducklings immunized with SL7207 (pVAX-UL24). Our study suggests that the SL7207 (pVAX-LTB-UL24) can be a candidate DEV vaccine.

摘要

口服 DNA 疫苗防治鸭肠炎病毒（DEV）的研究采用活减毒鼠伤寒沙门氏菌（SL7207）作为载体，大肠杆菌不耐热肠毒素 B 亚单位（LTB）作为黏膜佐剂。构建了 pVAX-UL24 和 pVAX-LTB-UL24 DNA 疫苗质粒，并转化入减毒鼠伤寒沙门氏菌 SL7207，分别得到 SL7207（pVAX-UL24）和 SL7207（pVAX-LTB-UL24）。经鸭口饲接种 SL7207（pVAX-UL24）或 SL7207（pVAX-LTB-UL24）后，记录抗 DEV 黏膜和系统免疫应答。为确定提供最大保护的最佳剂量，我们在口服免疫时使用了候选疫苗 SL7207（pVAX-LTB-UL24）的不同剂量。在 SL7207（pVAX-LTB-UL24）（1011 CFU）免疫组中，黏膜和系统免疫应答最强。因此，与 SL7207（pVAX-UL24）免疫组（40%）相比，SL7207（pVAX-LTB-UL24）口服免疫的雏鸭对致死性 DEV 攻毒的保护率（60-80%）更高。本研究表明，SL7207（pVAX-LTB-UL24）可以作为鸭肠炎病毒候选疫苗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94a8/3412168/c001fd9a2d53/1297-9716-43-56-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94a8/3412168/1977e08db0c9/1297-9716-43-56-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94a8/3412168/8f63add189c5/1297-9716-43-56-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94a8/3412168/856134868bea/1297-9716-43-56-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94a8/3412168/bc678926db8f/1297-9716-43-56-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94a8/3412168/002942ba7b9c/1297-9716-43-56-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94a8/3412168/c5357fc785ad/1297-9716-43-56-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94a8/3412168/c001fd9a2d53/1297-9716-43-56-7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94a8/3412168/1977e08db0c9/1297-9716-43-56-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94a8/3412168/8f63add189c5/1297-9716-43-56-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94a8/3412168/856134868bea/1297-9716-43-56-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94a8/3412168/bc678926db8f/1297-9716-43-56-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94a8/3412168/002942ba7b9c/1297-9716-43-56-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94a8/3412168/c5357fc785ad/1297-9716-43-56-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/94a8/3412168/c001fd9a2d53/1297-9716-43-56-7.jpg

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3
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