Hwang S G, Yano H, Kawashima R
Department of Animal Science, Faculty of Agriculture, Kyoto University, Japan.
J Nutr Sci Vitaminol (Tokyo). 1992 Apr;38(2):127-39. doi: 10.3177/jnsv.38.127.
The present study was undertaken to investigate the effects of dietary medium and long chain triglycerides (MCT and LCT) on fat deposition and fatty acid composition of adipose tissues of rats. Twenty-seven Wistar strain male rats were divided into three groups and fed semisynthetic experimental diets: Basal (AIN 76), MCT (basal+C8:0 10%), LCT (basal+corn oil 10%). Feed intake was measured every day and body weight was measured once a week. At the beginning, 4th and 8th week of experimental feeding, 3, 12, 12 rats were slaughtered, respectively. Liver, perirenal and epididymal adipose tissue pads were weighted and their fatty acid composition was determined. Chemical composition of wholebody carcass was measured. Body weight gain was greater in rats fed the LCT diet than in rats fed the basal and MCT diets. Feed intakes were decreased in rats fed the MCT and LCT diets compared to that of the basal group. Subsequently, when compared to the basal group rats, feed efficiencies in rats fed the MCT and LCT diets were improved at the level of 6 and 14% for 4 weeks, and then 17 and 24% for 8 weeks, respectively. The weight of perirenal and epididymal adipose tissue pads tended to be larger in rats fed the MCT and LCT diets than in the basal group, although not significant. There were not significant differences in wholebody composition among the three groups. But it appeared that in rats fed the MCT diet, moisture content tended to be decreased and crude protein content increased compared to the LCT group rats. Fatty acid composition of the rats fed the LCT diet showed a good reflection of dietary fatty acid composition. Unlike LCT, little of medium chain fatty acid (MCFA) itself were incorporated into liver or adipose tissues. However, it seems that fat deposition of the MCT group was accomplished not by incorporation of dietary fatty acids but by de novo fatty acids synthesis.
本研究旨在探讨膳食中链和长链甘油三酯（MCT和LCT）对大鼠脂肪组织脂肪沉积和脂肪酸组成的影响。将27只Wistar品系雄性大鼠分为三组，分别饲喂半合成实验日粮：基础日粮（AIN 76）、MCT日粮（基础日粮+C8:0 10%）、LCT日粮（基础日粮+玉米油10%）。每天测量采食量，每周测量一次体重。在实验喂养的第0周、第4周和第8周，分别宰杀3只、12只、12只大鼠。称量肝脏、肾周和附睾脂肪组织垫的重量，并测定其脂肪酸组成。测量全身体成分的化学组成。饲喂LCT日粮的大鼠体重增加量大于饲喂基础日粮和MCT日粮的大鼠。与基础组相比，饲喂MCT和LCT日粮的大鼠采食量降低。随后，与基础组大鼠相比，饲喂MCT和LCT日粮的大鼠在4周时的饲料效率分别提高了6%和14%，在8周时分别提高了17%和24%。饲喂MCT和LCT日粮的大鼠肾周和附睾脂肪组织垫的重量虽无显著差异，但有比基础组更大的趋势。三组大鼠的全身体成分无显著差异。但与LCT组大鼠相比，饲喂MCT日粮的大鼠水分含量有降低趋势，粗蛋白含量有增加趋势。饲喂LCT日粮的大鼠脂肪酸组成很好地反映了日粮脂肪酸组成。与LCT不同，中链脂肪酸（MCFA）本身很少掺入肝脏或脂肪组织。然而，MCT组的脂肪沉积似乎不是通过日粮脂肪酸掺入，而是通过脂肪酸从头合成来完成的。
