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Two RNA polymerase complexes from vesicular stomatitis virus-infected cells that carry out transcription and replication of genome RNA.来自感染水疱性口炎病毒的细胞的两种RNA聚合酶复合物，它们负责基因组RNA的转录和复制。
Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):5952-7. doi: 10.1073/pnas.0401449101. Epub 2004 Apr 6.
2
Basic amino acid residues at the carboxy-terminal eleven amino acid region of the phosphoprotein (P) are required for transcription but not for replication of vesicular stomatitis virus genome RNA.对于水疱性口炎病毒基因组RNA的转录而非复制而言，磷蛋白（P）羧基末端11个氨基酸区域的碱性氨基酸残基是必需的。
Virology. 1997 Nov 10;238(1):103-14. doi: 10.1006/viro.1997.8823.
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Unique capping activity of the recombinant RNA polymerase (L) of vesicular stomatitis virus: association of cellular capping enzyme with the L protein.水泡性口炎病毒重组RNA聚合酶（L）的独特加帽活性：细胞加帽酶与L蛋白的关联
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Transcription and replication initiate at separate sites on the vesicular stomatitis virus genome.转录和复制在水疱性口炎病毒基因组的不同位点起始。
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本文引用的文献

1
Identification of a novel tripartite complex involved in replication of vesicular stomatitis virus genome RNA.鉴定一种参与水疱性口炎病毒基因组RNA复制的新型三方复合物。
J Virol. 2003 Jan;77(1):732-8. doi: 10.1128/jvi.77.1.732-738.2003.
2
Identification of Hsp90 as a stimulatory host factor involved in influenza virus RNA synthesis.鉴定热休克蛋白90作为参与流感病毒RNA合成的刺激性宿主因子。
J Biol Chem. 2002 Nov 22;277(47):45306-14. doi: 10.1074/jbc.M206822200. Epub 2002 Sep 10.
3
Transcription and replication initiate at separate sites on the vesicular stomatitis virus genome.转录和复制在水疱性口炎病毒基因组的不同位点起始。
Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9178-83. doi: 10.1073/pnas.152155599. Epub 2002 Jun 27.
4
Unique capping activity of the recombinant RNA polymerase (L) of vesicular stomatitis virus: association of cellular capping enzyme with the L protein.水泡性口炎病毒重组RNA聚合酶（L）的独特加帽活性：细胞加帽酶与L蛋白的关联
Biochem Biophys Res Commun. 2002 Apr 26;293(1):264-8. doi: 10.1016/S0006-291X(02)00217-6.
5
Carboxy-terminal five amino acids of the nucleocapsid protein of vesicular stomatitis virus are required for encapsidation and replication of genome RNA.水泡性口炎病毒核衣壳蛋白的羧基末端五个氨基酸是基因组RNA包装和复制所必需的。
Virology. 1999 Jun 20;259(1):219-27. doi: 10.1006/viro.1999.9768.
6
Enhanced measles virus cDNA rescue and gene expression after heat shock.热休克后增强的麻疹病毒cDNA拯救及基因表达
J Virol. 1999 May;73(5):3560-6. doi: 10.1128/JVI.73.5.3560-3566.1999.
7
RNA polymerase of vesicular stomatitis virus specifically associates with translation elongation factor-1 alphabetagamma for its activity.水疱性口炎病毒的RNA聚合酶因其活性而与翻译延伸因子1αβγ特异性结合。
Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1449-54. doi: 10.1073/pnas.95.4.1449.
8
Basic amino acid residues at the carboxy-terminal eleven amino acid region of the phosphoprotein (P) are required for transcription but not for replication of vesicular stomatitis virus genome RNA.对于水疱性口炎病毒基因组RNA的转录而非复制而言，磷蛋白（P）羧基末端11个氨基酸区域的碱性氨基酸残基是必需的。
Virology. 1997 Nov 10;238(1):103-14. doi: 10.1006/viro.1997.8823.
9
Phosphorylation within the amino-terminal acidic domain I of the phosphoprotein of vesicular stomatitis virus is required for transcription but not for replication.水泡性口炎病毒磷蛋白氨基末端酸性结构域I内的磷酸化是转录所必需的，但不是复制所必需的。
J Virol. 1997 Nov;71(11):8167-75. doi: 10.1128/JVI.71.11.8167-8175.1997.
10
Initiation of vesicular stomatitis virus mutant polR1 transcription internally at the N gene in vitro.水泡性口炎病毒突变体polR1在体外于N基因处从内部起始转录。
J Virol. 1997 Feb;71(2):1466-75. doi: 10.1128/JVI.71.2.1466-1475.1997.

来自感染水疱性口炎病毒的细胞的两种RNA聚合酶复合物，它们负责基因组RNA的转录和复制。

Two RNA polymerase complexes from vesicular stomatitis virus-infected cells that carry out transcription and replication of genome RNA.

作者信息

Qanungo Kaustubha R, Shaji Daniel, Mathur Manjula, Banerjee Amiya K

机构信息

Virology Section, Department of Molecular Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA.

出版信息

Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):5952-7. doi: 10.1073/pnas.0401449101. Epub 2004 Apr 6.

DOI:10.1073/pnas.0401449101
PMID:15069200
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC395904/
Abstract

By immunoaffinity column chromatography, we have purified two RNA polymerase complexes, the transcriptase and replicase, from vesicular stomatitis virus-infected baby hamster kidney cells. The transcriptase is a multiprotein complex, containing the virus-encoded RNA polymerase L and P proteins, and two cellular proteins, translation elongation factor-1alpha and heat-shock protein 60. In addition, the complex contains a submolar amount of cellular mRNA cap guanylyltransferase. The replicase, on the other hand, is a complex containing the viral proteins, L, P, and the nucleocapsid (N), but lacking elongation factor-1alpha, heat-shock protein 60, and guanylyltransferase. The transcriptase complex synthesizes capped mRNAs and initiates transcription at the first gene (N) start site, whereas the replicase complex initiates RNA synthesis at the precise 3' end of the genome RNA and synthesizes encapsidated replication products in the presence of the N-P complex. We propose that two RNA polymerase complexes that differ in their content of virally and host-encoded proteins are separately responsible for transcription and replication of vesicular stomatitis virus genome RNA.

摘要

通过免疫亲和柱层析，我们从感染水疱性口炎病毒的幼仓鼠肾细胞中纯化出了两种RNA聚合酶复合物，即转录酶和复制酶。转录酶是一种多蛋白复合物，包含病毒编码的RNA聚合酶L和P蛋白，以及两种细胞蛋白，即翻译延伸因子-1α和热休克蛋白60。此外，该复合物还含有亚摩尔量的细胞mRNA帽鸟苷酸转移酶。另一方面，复制酶是一种包含病毒蛋白L、P和核衣壳（N）的复合物，但缺乏延伸因子-1α、热休克蛋白60和鸟苷酸转移酶。转录酶复合物合成带帽的mRNA，并在第一个基因（N）起始位点启动转录，而复制酶复合物在基因组RNA的精确3'端起始RNA合成，并在N-P复合物存在的情况下合成包裹的复制产物。我们提出，两种在病毒和宿主编码蛋白含量上不同的RNA聚合酶复合物分别负责水疱性口炎病毒基因组RNA的转录和复制。