氧化苦参碱对乙肝病毒转基因小鼠血清乙肝病毒DNA的抑制作用。
Inhibitory effect of oxymatrine on serum hepatitis B virus DNA in HBV transgenic mice.
作者信息
Lu Lun-Gen, Zeng Min-De, Mao Yi-Min, Fang Jing-Yuan, Song Yu-Lin, Shen Zhao-Hui, Cao Ai-Ping
机构信息
Shanghai Institute of Digestive Disease, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.
出版信息
World J Gastroenterol. 2004 Apr 15;10(8):1176-9. doi: 10.3748/wjg.v10.i8.1176.
AIM
To study the inhibitory effect of oxymatrine on serum hepatitis B virus (HBV) DNA in HBV transgenic mice.
METHODS
HBV transgenic mice model was established by microinjection, and identified by HBV DNA integration and replication. Transgenic mice with replicating HBV were divided into 3 groups, and injected with normal saline (group A, n=9), 50 mg/kg (group B, n=8) and 100 mg/kg (group C, n=9) oxymatrine intraperitoneally once a day for 30 d, respectively. Quantitation of serum HBV DNA in HBV transgenic mice was performed by competitive polymerase chain reaction (PCR) in combination with DNA hybridization quantitative detection technique before and after treatment.
RESULTS
Compared with pre-treatment, the serum HBV DNA in group A (F=1.04, P=0.9612) and group B (F=1.13, P=0.8739) had no changes after treatment. However, in group C serum HBV DNA was significantly decreased (F=13.97, P=0.0012). The serum HBV DNA after treatment was lower in group C than in groups B and A (F=8.65, P=0.0068; F=12.35, P=0.0018; respectively). The serum HBV DNA after treatment was lower in group B than in group A, but there was no statistical significance (F=1.43, P=0.652).
CONCLUSION
Oxymatrine has inhibitory effects on serum HBV DNA in HBV transgenic mice.
目的
研究氧化苦参碱对乙型肝炎病毒（HBV）转基因小鼠血清HBV DNA的抑制作用。
方法
通过显微注射建立HBV转基因小鼠模型，并通过HBV DNA整合与复制进行鉴定。将具有复制性HBV的转基因小鼠分为3组，分别腹腔注射生理盐水（A组，n = 9）、50 mg/kg氧化苦参碱（B组，n = 8）和100 mg/kg氧化苦参碱（C组，n = 9），每天1次，共30天。采用竞争性聚合酶链反应（PCR）结合DNA杂交定量检测技术，检测治疗前后HBV转基因小鼠血清HBV DNA含量。
结果
与治疗前相比，A组（F = 1.04，P = 0.9612）和B组（F = 1.13，P = 0.8739）治疗后血清HBV DNA无变化。然而，C组血清HBV DNA显著降低（F = 13.97，P = 0.0012）。治疗后C组血清HBV DNA低于B组和A组（分别为F = 8.65，P = 0.0068；F = 12.35，P = 0.0018）。治疗后B组血清HBV DNA低于A组，但差异无统计学意义（F = 1.43，P = 0.652）。
结论
氧化苦参碱对HBV转基因小鼠血清HBV DNA有抑制作用。
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