Lin M, Yang L Y, Li W Y, Peng Y P, Zheng Jia-Kun
Central Laboratory, Chaozhou Central Hospital, Chaozhou, Guangdong Province, China.
J Int Med Res. 2009 Sep-Oct;37(5):1411-9. doi: 10.1177/147323000903700515.
This study investigated the inhibitory capacity of oxymatrine on in vitro hepatitis B virus (HBV) replication. HepG2.2.15 cells were treated with oxymatrine 50, 100, 200, 400, 800 or 1000 mg/l, or with human interferon-alpha2b (IFN-alpha2b 1000 U/l) as a positive control. Levels of hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg) and HBV-DNA in cell supernatants were determined by enzyme-linked immunosorbent assay and fluorescent quantitative-polymerase chain reaction, respectively. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometric analysis and terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labelling were used to evaluate the cytotoxicity of oxymatrine. The inhibitory effects of oxymatrine gradually increased as the concentration increased from 200 to 1000 mg/l for HBsAg and HBeAg, and from 200 to 400 mg/l for HBV-DNA. There was no inhibitory effect of oxymatrine at concentrations < 200 mg/l. No significant difference was seen between human IFN-alpha2b (positive control) and oxymatrine >or= 200 mg/l. It is concluded that oxymatrine can inhibit in vitro HBV replication and antigen expression at concentrations >or= 200 mg/l.
本研究调查了氧化苦参碱对体外乙型肝炎病毒（HBV）复制的抑制能力。将HepG2.2.15细胞用50、100、200、400、800或1000mg/l的氧化苦参碱处理，或用人α2b干扰素（IFN-α2b 1000U/l）作为阳性对照。分别通过酶联免疫吸附测定和荧光定量聚合酶链反应测定细胞上清液中乙型肝炎表面抗原（HBsAg）、乙型肝炎e抗原（HBeAg）和HBV-DNA的水平。采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法、流式细胞术分析和末端脱氧核苷酸转移酶脱氧尿苷三磷酸缺口末端标记法评估氧化苦参碱的细胞毒性。对于HBsAg和HBeAg，氧化苦参碱的抑制作用随着浓度从200mg/l增加到1000mg/l而逐渐增强，对于HBV-DNA，浓度从200mg/l增加到400mg/l时抑制作用逐渐增强。氧化苦参碱在浓度<200mg/l时无抑制作用。人α2b干扰素（阳性对照）与氧化苦参碱≥200mg/l之间未见显著差异。结论是氧化苦参碱在浓度≥200mg/l时可抑制体外HBV复制和抗原表达。
