人血清中胰石蛋白的酶免疫测定及免疫化学特性分析

Enzyme immunoassay and immunochemical characterization of pancreatic stone protein in human serum.

作者信息

Katsuzaki T, Tatemichi N, Takeichi C, Hayakawa S, Hayakawa T, Shibata T, Nakae Y, Naruse S

机构信息

Department of Clinical Chemistry, Maruko Pharmaceutical Co., Ltd., Kasugai, Japan.

出版信息

J Clin Lab Anal. 1992;6(3):143-7. doi: 10.1002/jcla.1860060308.

DOI:10.1002/jcla.1860060308

PMID:1506981

Abstract

Monoclonal antibodies were raised against human pancreatic stone protein (PSP) and used for one-step enzyme immunoassay (EIA). PSP-S2-5 was employed as the standard in the assay. The assay's measurable range was 25-1,500 ng/ml and within run coefficient of variation was 3.7-6.4%. Analytical recovery of the assay was 101.5 +/- 5.65% (mean +/- SD). The results of experiments in which serum was fractionated by Mono S (cation exchange chromatography) suggested that most of immunoreactive material in human serum is PSP-S2-5. The EIA offers simple, rapid, and specific analysis of serum PSP level for clinical diagnosis.

摘要

制备了抗人胰石蛋白（PSP）的单克隆抗体，并将其用于一步酶免疫测定（EIA）。PSP-S2-5用作该测定中的标准品。该测定的可测量范围为25-1500 ng/ml，批内变异系数为3.7-6.4%。该测定的分析回收率为101.5 +/- 5.65%（平均值 +/- 标准差）。用Mono S（阳离子交换色谱法）对血清进行分级分离的实验结果表明，人血清中大部分免疫反应性物质是PSP-S2-5。该EIA为临床诊断提供了简单、快速且特异的血清PSP水平分析方法。