Podol'skiĭ I Ia, Shcheglov I V
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino State University, Pushchino.
Zh Vyssh Nerv Deiat Im I P Pavlova. 2004 Jan-Feb;54(1):59-67.
The effect of the maximum protein synthesis inhibition in brain and spinal cord on long-term memory formation in extreme situations was studied in various new behavioral tasks in rats. Cycloheximide injected bilaterally into the lateral ventricles three hours before learning suppressed protein synthesis in the central nervous system by 96% during one hour after learning. Forty-four hours after learning in a standard Morris water maze, the information about the platform position was not retained, whereas no memory disorder was observed in case of learning in a simplified Morris maze or a new test learned jump-out-of-water task. A more prolonged suppression of protein synthesis (76%, ten hours after learning) elicited amnesia in five out of eight rats learned in a simplified Morris maze but not disturbed information storage after 48 h and 14 days in the learned jump-out-of-water task. It was concluded that protein synthesis inhibitors are not a universal tool for disrupting formation of long-term memory. It was assumed that under extreme conditions, sometimes procedural long-term (to two weeks) memory is formed without de novo protein synthesis.
在大鼠的各种新行为任务中，研究了大脑和脊髓中最大程度的蛋白质合成抑制对极端情况下长期记忆形成的影响。在学习前3小时双侧向侧脑室注射环己酰亚胺，在学习后的1小时内可将中枢神经系统中的蛋白质合成抑制96%。在标准的莫里斯水迷宫中学习44小时后，关于平台位置的信息未被保留，而在简化的莫里斯迷宫中学习或在新的跳出水面任务中学习时，则未观察到记忆障碍。在简化的莫里斯迷宫中学习的8只大鼠中有5只在蛋白质合成受到更长时间抑制（学习后10小时抑制76%）时出现失忆，但在跳出水面任务中学习48小时和14天后，信息存储未受干扰。得出的结论是，蛋白质合成抑制剂不是破坏长期记忆形成的通用工具。据推测，在极端条件下，有时程序性长期（长达两周）记忆的形成无需从头合成蛋白质。
