Division of Diabetes, Digestive, and Kidney Diseases, Department of Clinical Molecular Medicine, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
Pharm Res. 2004 Mar;21(3):406-12. doi: 10.1023/B:PHAM.0000019292.03875.3e.
To evaluate chemosensitivity and its correlation with expression levels of the multidrug resistant transporter (MDR1) and the multidrug resistance-associated proteins 1 and 2 (MRP1, MRP2) mRNA in human colorectal adenocarcinomas.
Colorectal adenocarcinomas were obtained as surgical samples from 25 patients. The chemosensitivity of 12 anticancer drugs was assessed by the collagen gel droplet embedded culture drug sensitivity test (CD-DST). The expression levels of MDR1, MRP1, and MRP2 mRNA in colorectal adenocarcinomas were also evaluated by real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR).
The chemosensitivity was successfully evaluated for 16 of 25 patients, and the anticancer drugs were effective against the samples showing a relatively high growth rate. Gemcitabine hydrochloride was found to be more promising than those often prescribed for the treatment of colorectal adenocarcinoma. There was no correlation of the mRNA expression levels of MDR1 and MRP1 with the chemosensitivity of any anticancer drugs tested, but mitomycin C was found to be more effective for the colorectal adenocarcinoma with relatively high expression of MRP2 mRNA.
评估人结直肠腺癌的化疗敏感性及其与多药耐药转运蛋白（MDR1）和多药耐药相关蛋白 1 和 2（MRP1、MRP2）mRNA 表达水平的相关性。
从 25 例患者中获得结直肠腺癌的手术样本。通过胶原凝胶液滴嵌入培养药物敏感性试验（CD-DST）评估 12 种抗癌药物的化疗敏感性。通过实时定量逆转录聚合酶链反应（RT-PCR）评估结直肠腺癌中 MDR1、MRP1 和 MRP2 mRNA 的表达水平。
成功评估了 25 例患者中的 16 例，对生长速度相对较高的样本，抗癌药物有效。盐酸吉西他滨比常用于治疗结直肠腺癌的药物更有前途。MDR1 和 MRP1 mRNA 的表达水平与测试的任何抗癌药物的化疗敏感性均无相关性，但对于 MRP2 mRNA 表达水平相对较高的结直肠腺癌，丝裂霉素 C 更有效。
