Akomolafe R O, Adeoshun I O, Ayoka A O, Elujoba A A, Iwalewa E O
Department of Physiological Sciences, Obafemi Awolowo University, Ile-Ife, Nigeria.
Phytomedicine. 2004 Feb;11(2-3):249-54. doi: 10.1078/0944-7113-00330.
Folkloric evidence and scientific reports indicate the use of C. podocarpa fruit as a purgative recipe. This study attempts to find the in vitro effects of its aqueous infusion (ACPF) and methanolic extract (MCPF) on the motility of the intestine of albino rats of Wistar strain and to compare their effect with those of C. acutifolia fruit (ACAF and MCAF). MCPF relaxed both the ileum and colon dose dependently. Its effect was blocked by tolazoline (10(-9) M) and propranolol (10(-9) M). ACPF had no effect on the ileum, but contracted the colon dose-dependently. Its effect was blocked by nifedipine (2.8 x 10(-10) M) and drastically reduced by atropine (3.4 x 10(-6) M). MCAF has the same effect as ACPF on both ileum and colon and its effect was similarly affected by atropine (3.4 x 10(-6) M) and nifedipine (2.8 x 10(-8) M). ACAF relaxed the ileum, its effect was blocked by tolazoline (5.1 x 10(-7) M). MCAF was more potent than ACPF in contracting the colon, Hexamethonium (2.8 x 10(-8) M), chlorpheniramine (3.8 x 10(-8) M) and promethazine (3.2 x 10(-10) M) potentiated the effect of ACPF on the colon. The results suggest that both ACAF and MCPF have anti-diarrhoeal effect. MCPF acts via both alpha and beta adrenergic receptor stimulation, while ACAF stimulates alpha-receptor. ACPF and MCAF engage both the cholinergic system and calcium channel activation in causing purgation in the colon. The potentiation of the effect of ACPF by some blockers could be due to allosteric enhancement of the receptors involved in its action.
民间证据和科学报告表明，罗汉松果实被用作泻药配方。本研究旨在探讨其水浸液（ACPF）和甲醇提取物（MCPF）对Wistar品系白化大鼠肠道运动的体外作用，并将它们的作用与尖叶罗汉松果实（ACAF和MCAF）的作用进行比较。MCPF对回肠和结肠均有剂量依赖性的松弛作用。其作用被妥拉唑啉（10⁻⁹ M）和普萘洛尔（10⁻⁹ M）阻断。ACPF对回肠无作用，但对结肠有剂量依赖性的收缩作用。其作用被硝苯地平（2.8×10⁻¹⁰ M）阻断，并被阿托品（3.4×10⁻⁶ M）显著减弱。MCAF对回肠和结肠的作用与ACPF相同，其作用同样受到阿托品（3.4×10⁻⁶ M）和硝苯地平（2.8×10⁻⁸ M）的影响。ACAF使回肠松弛，其作用被妥拉唑啉（5.1×10⁻⁷ M）阻断。MCAF在收缩结肠方面比ACPF更有效，六甲铵（2.8×10⁻⁸ M）、氯苯那敏（3.8×10⁻⁸ M）和异丙嗪（3.2×10⁻¹⁰ M）增强了ACPF对结肠的作用。结果表明，ACAF和MCPF均有止泻作用。MCPF通过刺激α和β肾上腺素能受体发挥作用，而ACAF刺激α受体。ACPF和MCAF在引起结肠泻下时涉及胆碱能系统和钙通道激活。一些阻滞剂对ACPF作用的增强可能是由于其作用中涉及的受体的变构增强。
