Rousseau Bernard, Hirano Shigeru, Chan Roger W, Welham Nathan V, Thibeault Susan L, Ford Charles N, Bless Diane M
Department of Communicative Disorders, University of Wisconsin-Madison, Madison, Wisconsin 53705, USA.
J Voice. 2004 Mar;18(1):116-24. doi: 10.1016/j.jvoice.2003.06.001.
The purpose of the current study was to assess the histologic and rheologic properties of the scarred vocal fold lamina propria during a chronic phase of wound repair in a rabbit model. Eighteen rabbit larynges were scarred using a procedure that involved stripping the vocal fold lamina propria down to the thyroarytenoid muscle, using 3-mm microforceps. The approximate dimension of injury to the vocal fold was 3 x 1.5 x 0.5 mm [length x width x depth]. At 6 months postoperatively, histologic analysis of the scarred and control lamina propria in eight of these rabbits was completed for collagen, procollagen, elastin, and hyaluronic acid. Compared with control samples, scarred tissue samples revealed fragmented and disorganized elastin fibers. Additionally, collagen was significantly increased, organized, and formed thick bundles in the scarred vocal fold lamina propria. Measurements of the viscoelastic shear properties of the scarred and control lamina propria in the remaining 10 rabbits revealed increased elastic shear modulus (G') in 8 of 10 scarred samples and increased dynamic viscosity (eta') in 9 of 10 scarred samples. Although rheologic differences were not statistically significant, they revealed that on average, scarred samples were stiffer and more viscous than the normal controls. Histologic data are interpreted as indicating that by 6 months postinjury, the scarred rabbit vocal fold has reached a mature phase of wound repair, characterized by an increased, organized, and thick bundle collagen matrix. Rheologic data are interpreted as providing support for the potential role of increased, thick bundle collagen, and a disorganized elastin network on shear stiffness and dynamic viscosity in the chronic vocal fold scar. Based on these results, a 6-month postoperative time frame is proposed for future studies of chronic vocal fold scarring using the rabbit animal model.
本研究的目的是在兔模型的伤口修复慢性期评估瘢痕化声带固有层的组织学和流变学特性。使用3毫米微型镊子对18只兔的喉部进行瘢痕化处理，该操作包括将声带固有层剥离至甲状杓肌。声带损伤的大致尺寸为3×1.5×0.5毫米[长×宽×深]。术后6个月，对其中8只兔的瘢痕化和对照固有层进行了胶原、前胶原、弹性蛋白和透明质酸的组织学分析。与对照样本相比，瘢痕化组织样本显示弹性蛋白纤维破碎且排列紊乱。此外，瘢痕化声带固有层中的胶原显著增加、排列有序并形成粗束。对其余10只兔的瘢痕化和对照固有层的粘弹性剪切特性测量显示，10个瘢痕化样本中有8个弹性剪切模量（G'）增加，10个瘢痕化样本中有9个动态粘度（η'）增加。尽管流变学差异无统计学意义，但显示瘢痕化样本平均比正常对照更硬且更粘。组织学数据表明，损伤后6个月，瘢痕化兔声带已达到伤口修复的成熟阶段，其特征是胶原基质增加、排列有序且形成粗束。流变学数据为增加的粗束胶原和紊乱的弹性蛋白网络在慢性声带瘢痕的剪切刚度和动态粘度方面的潜在作用提供了支持。基于这些结果，提出术后6个月的时间框架，用于未来使用兔动物模型研究慢性声带瘢痕化。
