Hertegård S, Cedervall J, Svensson B, Forsberg K, Maurer F H J, Vidovska D, Olivius P, Ahrlund-Richter L, Le Blanc K
Department of Logopedics and Phoniatrics, Karolinska University Hospital, Huddinge, Stockholm, Sweden.
Laryngoscope. 2006 Jul;116(7):1248-54. doi: 10.1097/01.mlg.0000224548.68499.35.
OBJECTIVE/HYPOTHESIS: The aim of this study was to analyze the short-term viscoelastic and histologic properties of scarred rabbit vocal folds after injection of human mesenchymal stem cells (MSC) as well as the degree of MSC survival. Because MSCs are antiinflammatory and regenerate mesenchymal tissues, can MSC injection reduce vocal fold scarring after injury?
Twelve vocal folds from 10 New Zealand rabbits were scarred by a localized resection and injected with human MSC or saline. Eight vocal folds were left as controls.
After 4 weeks, 10 larynges were stained for histology and evaluation of the lamina propria thickness. Collagen type I content was analyzed from six rabbits. MSC survival was analyzed by fluorescent in situ hybridization staining from three rabbits. Viscoelasticity for 10 vocal folds was analyzed in a parallel-plate rheometer.
The rheometry on fresh-frozen samples showed decreased dynamic viscosity and lower elastic modulus (P<.01) in the scarred samples injected with MSC as compared with the untreated scarred group. Normal controls had lower dynamic viscosity and elastic modulus as compared with the scarred untreated and treated vocal folds (P<.01). Histologic analysis showed a higher content of collagen type 1 in the scarred samples as compared with the normal vocal folds and with the scarred folds treated with MSC. MSCs remained in all samples analyzed.
The treated scarred vocal folds showed persistent MSC. Injection of scarred rabbit vocal folds with MSC rendered improved viscoelastic parameters and less signs of scarring expressed as collagen content in comparison to the untreated scarred vocal folds.
目的/假设：本研究的目的是分析注射人骨髓间充质干细胞（MSC）后瘢痕化兔声带的短期粘弹性和组织学特性，以及MSC的存活程度。由于MSC具有抗炎作用并能再生间充质组织，那么注射MSC能否减少损伤后的声带瘢痕形成？
对10只新西兰兔的12条声带进行局部切除使其瘢痕化，然后注射人MSC或生理盐水。8条声带留作对照。
4周后，对10个喉部进行组织学染色并评估固有层厚度。分析6只兔的Ⅰ型胶原含量。通过荧光原位杂交染色分析3只兔的MSC存活情况。在平行板流变仪中分析10条声带的粘弹性。
对新鲜冷冻样本的流变学测量显示，与未治疗的瘢痕化组相比，注射MSC的瘢痕化样本的动态粘度降低，弹性模量降低（P<0.01）。与未治疗和治疗后的瘢痕化声带相比，正常对照组的动态粘度和弹性模量较低（P<0.01）。组织学分析显示，与正常声带以及用MSC治疗的瘢痕化声带相比，瘢痕化样本中的Ⅰ型胶原含量更高。在所有分析的样本中均发现有MSC存在。
治疗后的瘢痕化声带中存在持续的MSC。与未治疗的瘢痕化声带相比，向瘢痕化兔声带注射MSC可改善粘弹性参数，并减少以胶原含量表示的瘢痕形成迹象。
