Soares Caroline C, Volotão Eduardo M, Albuquerque Maria Carolina M, Nozawa Carlos M, Linhares Rosa Elisa C, Volokhov Dmitriy, Chizhikov Vladimir, Lu Xiaoyan, Erdman Dean, Santos Norma
Departamento de Virologia, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-590, Brazil.
J Clin Microbiol. 2004 Apr;42(4):1723-6. doi: 10.1128/JCM.42.4.1723-1726.2004.
Single-stranded conformation polymorphism (SSCP) analysis and heteroduplex mobility assays (HMAs) were used to identify and genotype enteric adenoviruses (EAd). The results were compared to those of restriction endonuclease assays, species-specific PCRs, and direct nucleotide sequence analyses. Of the 31 stool samples tested, 15 isolates were identified as EAd and 7 were identified as nonenteric Ad by all methods. An agreement of 100% was found between the SSCP and HMA results.
采用单链构象多态性（SSCP）分析和异源双链迁移率分析（HMA）对肠道腺病毒（EAd）进行鉴定和基因分型。将结果与限制性内切酶分析、种特异性聚合酶链反应（PCR）以及直接核苷酸序列分析的结果进行比较。在所检测的31份粪便样本中，所有方法均鉴定出15株分离株为EAd，7株为非肠道腺病毒（Ad）。SSCP和HMA结果之间的一致性为100%。
