通过多重聚合酶链反应检测法对人腺病毒进行种属特异性鉴定。
Species-specific identification of human adenoviruses by a multiplex PCR assay.
作者信息
Xu W, McDonough M C, Erdman D D
机构信息
Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
出版信息
J Clin Microbiol. 2000 Nov;38(11):4114-20. doi: 10.1128/JCM.38.11.4114-4120.2000.
Abstract
A multiplex PCR assay was developed by using primers to the fiber gene that could differentiate human adenovirus (Ad) species A through F in a single amplification reaction. The assay correctly identified the species of all 49 recognized Ad prototype strains as well as 180 geographically and temporally diverse Ad field isolates. Ad serotype 6 (Ad6) (species C), Ad16 (species B), Ad31 (species A), and Ad40 and Ad41 (species F) could also be distinguished by amplicon size within each respective species. In comparison, a previously described Ad species-specific multiplex PCR assay that used primers to the Ad hexon gene gave equivocal results with several serotypes of species B, whereas our multiplex assay amplified all species B serotypes equally well. Our multiplex PCR assay will permit rapid, accurate, and cost-effective classification of Ad isolates.
摘要
通过使用针对纤维基因的引物开发了一种多重PCR检测方法，该方法可在单个扩增反应中区分人腺病毒（Ad）A至F种。该检测方法正确鉴定了所有49种公认的Ad原型菌株以及180株来自不同地理和时间的Ad野外分离株的种类。腺病毒血清型6（Ad6）（C种）、Ad16（B种）、Ad31（A种）以及Ad40和Ad41（F种）也可通过各自种内的扩增子大小进行区分。相比之下，先前描述的一种使用针对腺病毒六邻体基因的引物的腺病毒种特异性多重PCR检测方法，对几种B种血清型给出了模棱两可的结果，而我们的多重检测方法对所有B种血清型的扩增效果同样良好。我们的多重PCR检测方法将允许对腺病毒分离株进行快速、准确且经济高效的分类。
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