Fujitake Shinichi, Hibi Kenji, Okochi Osamu, Kodera Yasuhiro, Ito Katsuki, Akiyama Seiji, Nakao Akimasa
Gastrointestinal Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
J Gastroenterol. 2004;39(2):120-4. doi: 10.1007/s00535-003-1262-0.
BACKGROUND Recently, it was demonstrated that sup-pressor of cytokine signaling-1 (SOCS-1) was frequently silenced by methylation of its CpG island inhuman hepatocellular carcinoma (HCC). To define the role of SOCS-1 in the tumorigenic pathway of the colorectum, we examined the methylation of SOCS-1 in tumors of colorectal cancer patients.
We examined 74 colorectal cancer patients, using a methylation-specific polymerase chain reaction (PCR;MSP) for SOCS-1 CpG island in primary tumors.
Aberrant methylation of the SOCS-1 CpG island was detected in 6 of the 74 (8%) colorectal cancer specimens. No corresponding normal colorectal tissues showed SOCS-1 methylation. We then analyzed the correlation between the clinicopathological features and SOCS-1 aberrant methylation and found that younger age was significantly related to SOCS-1 methylation (P = 0.048).
These findings suggested that SOCS-1 may act as a tumor suppressor in at least some colorectal cancers and that SOCS-1 methylation may be a particular phenomenon related to a nearly onset of colorectal cancer.
背景 最近，有研究表明，细胞因子信号转导抑制因子1（SOCS-1）在人类肝细胞癌（HCC）中常因其CpG岛甲基化而沉默。为了明确SOCS-1在结直肠癌致瘤途径中的作用，我们检测了结直肠癌患者肿瘤中SOCS-1的甲基化情况。
我们对74例结直肠癌患者进行了研究，采用甲基化特异性聚合酶链反应（PCR；MSP）检测原发性肿瘤中SOCS-1的CpG岛。
在74例结直肠癌标本中的6例（8%）检测到SOCS-1 CpG岛的异常甲基化。相应的正常结直肠组织未显示SOCS-1甲基化。然后我们分析了临床病理特征与SOCS-1异常甲基化之间的相关性，发现年龄较小与SOCS-1甲基化显著相关（P = 0.048）。
这些发现提示，SOCS-1可能至少在某些结直肠癌中起肿瘤抑制作用，且SOCS-1甲基化可能是与结直肠癌发病相关的一种特殊现象。
