Su Meng, Qin Baoli, Liu Fang, Chen Yuze, Zhang Rui
Department of Medical Oncology, Liaoning Cancer Hospital and Institute, Cancer Hospital of China Medical University, Shenyang, Liaoning 110042, P.R. China.
Department of Colorectal Surgery, Liaoning Cancer Hospital and Institute, Cancer Hospital of China Medical University, Shenyang, Liaoning 110042, P.R. China.
Oncol Lett. 2018 Jul;16(1):65-72. doi: 10.3892/ol.2018.8645. Epub 2018 May 7.
The aim of the present study was to investigate the role of microRNA (miR)-885-5p in colorectal cancer cell proliferation and migration, and to determine the possible underlying molecular mechanisms. The expression of miR-885-5p in colorectal cancer tissue and cells was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The expression levels of three suppressor of cytokine signaling (SOCS) factors were detected by RT-qPCR and western blotting. The effects of miR-885-5p on tumor cell proliferation and migration were studied using MTT and Transwell assays, respectively. Additionally, the expression levels of epithelial-mesenchymal transition (EMT)-related proteins (N-cadherin, E-cadherin, vimentin and Snail) were detected by RT-qPCR and western blot analysis. Furthermore, the target of miR-885-5p was predicted and confirmed using a luciferase reporter assay. miR-885-5p was demonstrated to be upregulated and SOCS was downregulated in colorectal cancer tissue, and cells. miR-885-5p suppression significantly inhibited tumor cell proliferation and migration, promoted E-cadherin expression, and inhibited the expression levels of N-cadherin, vimentin and Snail. Further studies showed that SOCS5, SOCS6 and SOCS7 were direct targets of miR-885-5p. The results suggest that miR-885-5p suppression inhibited cell proliferation and migration, and the EMT process by targeting SOCS5, SOCS6 and SOCS7 genes in colorectal cancer. miR-885-5p and SOCS may be used for the diagnosis and treatment of colorectal cancer.
本研究旨在探讨微小RNA（miR）-885-5p在结直肠癌细胞增殖和迁移中的作用，并确定其可能的潜在分子机制。采用逆转录-定量聚合酶链反应（RT-qPCR）检测结直肠癌组织和细胞中miR-885-5p的表达。通过RT-qPCR和蛋白质免疫印迹法检测三种细胞因子信号转导抑制因子（SOCS）的表达水平。分别采用MTT法和Transwell实验研究miR-885-5p对肿瘤细胞增殖和迁移的影响。此外，通过RT-qPCR和蛋白质免疫印迹分析检测上皮-间质转化（EMT）相关蛋白（N-钙黏蛋白、E-钙黏蛋白、波形蛋白和Snail）的表达水平。此外，使用荧光素酶报告基因检测法预测并证实miR-885-5p的靶标。结果表明，在结直肠癌组织和细胞中，miR-885-5p上调而SOCS下调。抑制miR-885-5p可显著抑制肿瘤细胞增殖和迁移，促进E-钙黏蛋白表达，并抑制N-钙黏蛋白、波形蛋白和Snail的表达水平。进一步研究表明，SOCS5、SOCS6和SOCS7是miR-885-5p的直接靶标。结果提示，在结直肠癌中，抑制miR-885-5p通过靶向SOCS5、SOCS6和SOCS7基因抑制细胞增殖、迁移及EMT过程。miR-885-5p和SOCS可能用于结直肠癌的诊断和治疗。
