Li Xilong, Zhao Xinxu
Department of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu 730070, PR China.
Vet Res Commun. 2004 Apr;28(3):235-45. doi: 10.1023/b:verc.0000017370.74401.be.
Ovulation in the Bactrian camel depends upon ovulation-inducing factors in the seminal plasma. The present study was conducted to isolate and purify the bioactive fractions from the seminal plasma of these camels. The seminal plasma was fractionated by anion-exchange chromatography, and six fractions were obtained. The bioactive potential of each fraction was estimated from its effect on rat pituitary tissue cultured in vitro and by the effect of an intramuscular injection of the fraction into female camels in vivo. Both the third fraction (F3) and the fifth fraction (F5) stimulated the release of LH in vitro and in vivo. In addition, female camels ovulated within 48 h after intramuscular injection of F3. However, neither F3 nor F5 had any significant effect on the secretion of FSH, either in vitro or in vivo. When F3 was further fractionated into four subfractions, the third subfraction (F3-3) still stimulated the in vitro release of LH, but not of FSH. An attempt to further purify the ovulation-inducing factors in F3-3 failed owing to the similarity of the molecular characters.
双峰驼的排卵取决于精浆中的排卵诱导因子。本研究旨在从这些骆驼的精浆中分离和纯化生物活性成分。通过阴离子交换色谱法对精浆进行分级分离，得到了六个级分。通过各组分对体外培养的大鼠垂体组织的作用以及将该组分肌肉注射到雌性骆驼体内的作用，评估了每个级分的生物活性潜力。第三级分（F3）和第五级分（F5）在体外和体内均刺激了促黄体生成素（LH）的释放。此外，雌性骆驼在肌肉注射F3后48小时内排卵。然而，F3和F5在体外或体内对促卵泡生成素（FSH）的分泌均无显著影响。当F3进一步分为四个亚级分时，第三个亚级分（F3-3）仍能刺激体外LH的释放，但不能刺激FSH的释放。由于分子特征相似，进一步纯化F3-3中排卵诱导因子的尝试失败了。
