al-Somai N, Vishwanath R, Shannon P, Molan P C
Department of Biological Sciences, University of Waikato, Hamilton, New Zealand.
Reprod Fertil Dev. 1994;6(2):165-71. doi: 10.1071/rd9940165.
Dialysis of diluted semen before cryopreservation is beneficial to sperm survival. This is due to removal of low molecular weight components from seminal plasma that are damaging to sperm. The apparent molecular weights (M(r)) of these components range between 1000 and 12,000 as estimated by gel permeation chromatography and electrophoresis. The detrimental effect on sperm motility is greatest with the components of M(r) between 5000 and 12,000 and with those of M(r) < 1500. Their effect on sperm motility was dependent on concentration. The small molecular weight components were derived from the high molecular weight components of seminal plasma through disaggregation under prescribed conditions.
精液冷冻保存前对稀释后的精液进行透析有利于精子存活。这是因为从精浆中去除了对精子有损害的低分子量成分。通过凝胶渗透色谱法和电泳估计，这些成分的表观分子量（M(r)）在1000至12,000之间。分子量在5000至12,000之间以及分子量<1500的成分对精子活力的有害影响最大。它们对精子活力的影响取决于浓度。小分子成分是在规定条件下通过精浆高分子量成分的解聚产生的。
