Schroder L, Basta N T, Casteel S W, Evans T J, Payton M E, Si J
Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK 74078, USA.
J Environ Qual. 2004 Mar-Apr;33(2):513-21. doi: 10.2134/jeq2004.5130.
The effect of the dosing vehicle (e.g., dough) on the ability of an in vitro gastrointestinal (IVG) method to predict relative bioavailable Pb associated with soil ingestion was evaluated. Bioaccessible Pb determined by the IVG method was compared with relative bioavailable Pb measured from dosing trials using juvenile swine for 18 contaminated soils ranging from 1270 to 14200 mg Pb kg(-1). Bioaccessible Pb was measured in the IVG gastric extraction (GE) and intestinal extraction (IE) solutions. Mean bioaccessible Pb values were 32.2% for GE without dough, 23.0% for GE with dough, 1.06% for IE without dough, and 0.56% for IE with dough. It is possible that phytic acid associated with the dough addition decreased bioaccessible Pb. In vivo relative bioavailable Pb ranges for different swine tissues were 1 to 87% for blood, 0 to 110% for liver, 1 to 124% for kidney, and 0.04 to 94% for bone. Strong linear relationships between IVG GE Pb with dough (r > 0.76, P < 0.0002), IVG IE Pb with dough (r > 0.56, P < 0.015), and IVG GE Pb without dough (r > 0.81, P < 0.0001) and in vivo bioavailable Pb as estimated with blood, kidney, liver, and bone were found. Inexpensive in vitro methods may be useful in providing an estimate of the variability in relative bioavailable Pb at a single study site. The IVG method can be used to estimate relative bioavailable Pb, As, and Cd in contaminated soil.
评估了给药载体（如面团）对体外胃肠道（IVG）方法预测与土壤摄入相关的相对生物可利用铅能力的影响。将通过IVG方法测定的生物可及性铅与使用幼猪对18种污染土壤（铅含量范围为1270至14200 mg Pb kg⁻¹）进行给药试验测得的相对生物可利用铅进行了比较。在IVG胃提取物（GE）和肠提取物（IE）溶液中测量生物可及性铅。不含面团的GE中生物可及性铅的平均含量为32.2%，含面团的GE中为23.0%，不含面团的IE中为1.06%，含面团的IE中为0.56%。添加的面团中含有的植酸可能降低了生物可及性铅。不同猪组织的体内相对生物可利用铅范围为：血液中为1%至87%，肝脏中为0%至110%，肾脏中为1%至124%，骨骼中为0.04%至94%。发现含面团的IVG GE铅（r > 0.76，P < 0.0002）、含面团的IVG IE铅（r > 0.56，P < 0.015）、不含面团的IVG GE铅（r > 0.81，P < 0.0001）与通过血液、肾脏、肝脏和骨骼估算的体内生物可利用铅之间存在强线性关系。廉价的体外方法可能有助于估计单个研究地点相对生物可利用铅的变异性。IVG方法可用于估计污染土壤中相对生物可利用的铅、砷和镉。
