Program in Molecular and Integrative Physiological Sciences, Department of Environmental Health, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA.
Environ Pollut. 2013 Nov;182:217-24. doi: 10.1016/j.envpol.2013.07.013. Epub 2013 Aug 7.
We correlated mineralogical and particle characteristics of Zn-containing particles with Zn geoavailability, bioaccessibility, and bioavailability following gavage and intranasal (IN) administration in rats. We compared samples of Zn/Pb mine waste and five pulverized pure-phase Zn minerals (<38 μm). Particles were neutron-activated to produce radioactive (65)Zn. We assessed geoavailability using sequential extractions and bioaccessibility using in vitro extraction tests simulating various pH and biological conditions. Zn in vivo bioavailability and in vitro bioaccessibility decreased as follows: mine waste > hydrozincite > hemimorphite > zincite ≈ smithsonite >> sphalerite. We found significant correlations among geoavailability, bioaccessibility and bioavailability. In particular, Zn bioavailability post-gavage and post-IN was significantly correlated with bioaccessibility in simulated phagolysosomal fluid and gastric fluid. These data indicate that solid phase speciation influences biological uptake of Zn and that in vitro tests can be used to predict Zn bioavailability in exposure assessment and effective remediation design.
我们将含锌颗粒的矿物学和颗粒特征与大鼠灌胃和鼻腔内（IN）给药后锌的生物利用度、生物可及性和生物利用度进行了关联。我们比较了 Zn/Pb 矿废物和五种粉碎的纯相 Zn 矿物质（<38 μm）的样本。用中子激活产生放射性（65）Zn。我们使用顺序提取评估生物可利用度，并使用模拟各种 pH 值和生物条件的体外提取试验评估生物可及性。体内 Zn 生物利用度和体外生物可及度依次降低：矿废物>水锌矿>纤锌矿>氧化锌≈菱锌矿>>闪锌矿。我们发现生物可利用度、生物可及性和生物可利用度之间存在显著相关性。特别是，灌胃和 IN 给药后 Zn 的生物利用度与模拟吞噬溶酶体液和胃液中的生物可及性显著相关。这些数据表明，固相形态影响 Zn 的生物摄取，体外试验可用于预测暴露评估和有效修复设计中的 Zn 生物利用度。
